Difference between revisions of "Part:BBa K3215017"

Revision as of 23:43, 19 October 2019

phnC Promoter homology

This part is a 50bp homologous sequence that occurs at region -35 of the phnC promoter from E. coli K-12. It can be used together with phnC UTR 5' homology part (BBa_K3215018) to form a construct containing a flanking region that changes the phn Operon promoter region through recombination techniques, removing the original one (e.g. BBa_K3215007).

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 33
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 33
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 33
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 33
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 33
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3215017 short</partinfo>
-This part is a 50bp homologous sequence that occurs at region -35 of the phnC promoter from E. coli K-12. It can be used together with phnC UTR 5' homology part (<partinfo>BBa_K3215018</partinfo>) to form a construct containing a flanking region to change the phn Operon promoter region through recombination techniques, removing the original one (e.g. <partinfo>BBa_K3215007</partinfo>).
+This part is a 50bp homologous sequence that occurs at region -35 of the phnC promoter from E. coli K-12. It can be used together with phnC UTR 5' homology part (<partinfo>BBa_K3215018</partinfo>) to form a construct containing a flanking region that changes the phn Operon promoter region through recombination techniques, removing the original one (e.g. <partinfo>BBa_K3215007</partinfo>).
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