Difference between revisions of "Part:BBa K3215008"

Latest revision as of 23:40, 19 October 2019

CmR Cassette + Medium Promoter

This part is composed of a selectable marker cassete (Chloramphenicol Resistance) and a medium promoter, together with a flanking region that enables the user to change the phn Operon promoter, utilizing a recombination technique.

The major advantage of this part is that the user can remove the selection marker later, using a recombination system, since the CmR Cassette used in this part (BBa_K3215006) contains a 48bp sequence that can be used as a homology source for recombinases.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 33
Illegal XbaI site found at 852
Illegal XbaI site found at 922
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 33
Illegal NheI site found at 1345
Illegal NheI site found at 1368
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 33
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 33
Illegal XbaI site found at 852
Illegal XbaI site found at 922
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 33
Illegal XbaI site found at 852
Illegal XbaI site found at 922
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3215008 short</partinfo>
-This part is composed of a selectable marker cassete (Chloramphenicol Resistance) and a medium promoter, together with a flanking region that enables the user to change the phn Operon promoter using a recombination technique.
+This part is composed of a selectable marker cassete (Chloramphenicol Resistance) and a medium promoter, together with a flanking region that enables the user to change the phn Operon promoter, utilizing a recombination technique.
 The major advantage of this part is that the user can remove the selection marker later, using a recombination system, since the CmR Cassette used in this part (<partinfo>BBa_K3215006</partinfo>) contains a 48bp sequence that can be used as a homology source for recombinases.