Generator

Part:BBa_K3215006

Designed by: Daniel Oppermann Peixoto   Group: iGEM19_UFRGS_Brazil   (2019-09-07)


CmR Cassette

The utilization of this part enables the user to add a chloramphenicol resistance gene next to a short 48bp sequence from S cerevisiae in any vector desired. The importance of the latter sequence is to facilitate further removal of this cassette by recombination, if it has been inserted into the genomic sequence.

This part can be used as a selectable marker in any system, independently of the 48bp sequence, although it can cause some trouble if the organism being used is the same as the one which the 48bp sequence came from.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 794
    Illegal XbaI site found at 864
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 794
    Illegal XbaI site found at 864
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 794
    Illegal XbaI site found at 864
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None