Difference between revisions of "Part:BBa K3037003"

Revision as of 21:39, 17 October 2019

Fusion protein dCas9 + HRP (MBP/dCas9/linker/HRP/Strep-tag)

Overview

The TU Dresden 2019 team design this biobrick in order to create a system for the short and quick detection of specific DNA sequences.

The plasmid used for the expression of the construct was pSB1C3.

Characterization

Outline

We performed the following characterization experiments:

1) Expression in pOOC97 (BBa_K3037000):

Experiments in Detail

1) Expression in pOOC97 (BBa_K3037000):

The fusion protein was expressed using as a backbone the plasmid pOOC97 (BB_aK3037000)

The purpose of this experiment is to show that the Escherichia coli grows normaly after the induction of the expression of the fusion protein.

For this the development of the culture was monitored by measuring the OD at 600 nm during different time before and after induction with 1 mM IPTG. As shown in the curve the growing of the bacteria is not affected by the expression of the protein.

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Sequence