Difference between revisions of "Part:pSB1A10"
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==References== | ==References== | ||
<biblio> | <biblio> | ||
+ | #Bernard-Gene-1999 pmid=7926841 | ||
#Khlebnikov pmid=12080425 | #Khlebnikov pmid=12080425 | ||
#Smolke pmid=12402322 | #Smolke pmid=12402322 | ||
</biblio> | </biblio> |
Latest revision as of 15:36, 10 March 2010
Screening Plasmid v1.0
This plasmid can be used to do a rough characterization of the Input/Output curve for a PoPS-based device. Additionally, it can be used for screening of part or device libaries via FACS. More information can be [http://openwetware.org/index.php?title=Endy:Screening_plasmid_1.0 found here.] Note that this plasmid contains ccdb, so should be transformed into Part:BBa_V1005, or another compatible strain for initial preparation.
Note: The sequence below is incorrect because the backbone that the SP device was inserted into was psb1A2 rather than psb1A3, so there is a terminator (not annotated) in the sequence below at position 1044-1121 that isn't actually present in the DNA of psb1A10. Updating this will take some time because I need to change all the feature locations by hand - but the Vector NTI file is correct.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 5242
Illegal NheI site found at 4443
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9
Illegal NotI site found at 5248 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 5242
Illegal BglII site found at 89
Illegal BamHI site found at 4382
Illegal XhoI site found at 67 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found at 5242
Illegal suffix found at 2 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found at 5242
Plasmid lacks a suffix.
Illegal XbaI site found at 5257
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal AgeI site found at 674
Illegal AgeI site found at 786
Illegal AgeI site found at 4217 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI.rc site found at 2197
Illegal BsaI.rc site found at 5118
Illegal SapI site found at 4199
References
<biblio>
- Bernard-Gene-1999 pmid=7926841
- Khlebnikov pmid=12080425
- Smolke pmid=12402322
</biblio>