Difference between revisions of "Part:BBa K2922038"
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===Summary=== | ===Summary=== | ||
| − | This is a composite part consist of a T7 promoter (<partinfo>BBa_K525998</partinfo>), CDS of colicin-N (<partinfo>BBa_K2922027</partinfo>), CDS of colicin-N immunity protein (<partinfo>BBa_K2922028</partinfo>), CDS of lysis protein (<partinfo>BBa_K2922029</partinfo>) and a gfasPurple chromoprotein reporter(<partinfo>BBa_K1033917</partinfo>) . Each CDS has an RBS (<partinfo>BBa_B0034</partinfo>) behind. T7 promoter could be induced by IPTG or lactose in Escherichia coli BL21 strain and then express all proteins mentioned. E coli that can`t express colicin-N immunity protein would be killed by colicin-N, colicin-N immunity protein is used to protect itself from attack of extracellular colicin-N and lysis protein helps the release of colicin-N. The gfasPurple chromoprotein reporter is used to present the growth curve specifically of strain which contains this part. This part is constructed in the aim of achieve our “aggressive” design. | + | This is a composite part consist of a T7 promoter (<partinfo>BBa_K525998</partinfo>), CDS of colicin-N (<partinfo>BBa_K2922027</partinfo>), CDS of colicin-N immunity protein (<partinfo>BBa_K2922028</partinfo>), CDS of lysis protein (<partinfo>BBa_K2922029</partinfo>) and a gfasPurple chromoprotein reporter(<partinfo>BBa_K1033917</partinfo>) . Each CDS has an RBS (<partinfo>BBa_B0034</partinfo>) behind. T7 promoter could be induced by IPTG or lactose in <i>Escherichia coli</i> BL21 strain and then express all proteins mentioned. <i>E coli</i> that can`t express colicin-N immunity protein would be killed by colicin-N, colicin-N immunity protein is used to protect itself from attack of extracellular colicin-N and lysis protein helps the release of colicin-N. The gfasPurple chromoprotein reporter is used to present the growth curve specifically of strain which contains this part. This part is constructed in the aim of achieve our “aggressive” design. |
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===Ientification=== | ===Ientification=== | ||
| − | <table><tr><th>[[Image:TND.jpg|thumb|Fig.1 The growth curve of E coli BL21 strain which contained BBa_K2922038 carried by pSB1C3. OD600, OD577 and OD458 is detected to specifically show its growth status]]</th><th></table> | + | <table><tr><th>[[Image:TND.jpg|thumb|Fig.1 The growth curve of <i>E coli</i> BL21 strain which contained BBa_K2922038 carried by pSB1C3. OD600, OD577 and OD458 is detected to specifically show its growth status]]</th><th></table> |
| + | <br> | ||
| + | <table><tr><th>[[Image:+TND.png|Fig.2 The co-cultural growth curve of <i>E coli</i> BL21 strains which contained BBa_K2922038 or BBa_K1033914. OD600, OD577 and OD458 is detected to specifically show each strans` growth status]]</th><th></table> | ||
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<partinfo>BBa_K2922038 SequenceAndFeatures</partinfo> | <partinfo>BBa_K2922038 SequenceAndFeatures</partinfo> | ||
Revision as of 15:18, 15 October 2019
The colicin-N operon under T7 promoter control with a gfasPurple chromoprotein reporter
Summary
This is a composite part consist of a T7 promoter (BBa_K525998), CDS of colicin-N (BBa_K2922027), CDS of colicin-N immunity protein (BBa_K2922028), CDS of lysis protein (BBa_K2922029) and a gfasPurple chromoprotein reporter(BBa_K1033917) . Each CDS has an RBS (BBa_B0034) behind. T7 promoter could be induced by IPTG or lactose in Escherichia coli BL21 strain and then express all proteins mentioned. E coli that can`t express colicin-N immunity protein would be killed by colicin-N, colicin-N immunity protein is used to protect itself from attack of extracellular colicin-N and lysis protein helps the release of colicin-N. The gfasPurple chromoprotein reporter is used to present the growth curve specifically of strain which contains this part. This part is constructed in the aim of achieve our “aggressive” design.
Ientification
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- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1938
Illegal NheI site found at 1961 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 598
Illegal AgeI site found at 1602 - 1000COMPATIBLE WITH RFC[1000]