Difference between revisions of "Part:BBa K3037003"
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The plasmid used for the expression of the construct was pSB1C3.
 
The plasmid used for the expression of the construct was pSB1C3.
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== Characterization ==
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=== Outline ===
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We performed the following characterization experiments:
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1) Expression in pOOC97 [https://parts.igem.org/Part:BBa_K3037000 (BBa_K3037000):]
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=== Experiments in Detail ===
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==== 1) Expression in pOOC97 [https://parts.igem.org/Part:BBa_K3037000 (BBa_K3037000):] ====
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The fusion protein was expressed using as a backbone the plasmid pOOC97 [https://parts.igem.org/Part:BBa_K3037000 (BB_aK3037000)]
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The purpose of this experiment is to show that the <span style="font-style: italic;">Escherichia coli</span> grows normaly after the induction of the expression of the fusion protein.
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For this the development of the culture was monitored by measuring the OD at 600 nm during different time before and after induction with 1 mM IPTG. As shown in the curve the growing of the bacteria is not affected by the expression of the protein.
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[[File:T--TU_Dresden--Expression_in_pOCC97_BBa_K3037003.png|500px|thumb|left|alt text]]
  
  

Revision as of 21:38, 17 October 2019

Fusion protein dCas9 + HRP (MBP/dCas9/linker/HRP/Strep-tag)

Fusion protein
Function Colour detection of specific DNA sequences
Use in Escherichia coli
RFC standard RFC 25 compatible
Backbone pSB1C3
Submitted by Team:TU_Dresden 2019[1]


Overview

The TU Dresden 2019 team design this biobrick in order to create a system for the short and quick detection of specific DNA sequences.

The plasmid used for the expression of the construct was pSB1C3.


Characterization

Outline

We performed the following characterization experiments:

1) Expression in pOOC97 (BBa_K3037000):

Experiments in Detail

1) Expression in pOOC97 (BBa_K3037000):

The fusion protein was expressed using as a backbone the plasmid pOOC97 (BB_aK3037000)

The purpose of this experiment is to show that the Escherichia coli grows normaly after the induction of the expression of the fusion protein.

For this the development of the culture was monitored by measuring the OD at 600 nm during different time before and after induction with 1 mM IPTG. As shown in the curve the growing of the bacteria is not affected by the expression of the protein.

alt text


Sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2302
    Illegal NheI site found at 5500
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 381
    Illegal BglII site found at 5742
    Illegal BamHI site found at 4581
    Illegal BamHI site found at 5314
    Illegal XhoI site found at 5826
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 79