Difference between revisions of "Part:BBa K2922035"

Revision as of 17:05, 14 October 2019

The colicin-N operon under pBAD(Arabinose promoter) control

Summary

This is a composite part consist of an arabinose promoter (BBa_K206000), CDS of colicin-N (BBa_K2922027), CDS of colicin-N immunity protein (BBa_K2922028) and CDS of lysis protein (BBa_K2922029). Each CDS has an RBS (BBa_B0034) behind. Arabinose promoter could be induced by Arabinose in Escherichia coli BL21 strain and then express all proteins mentioned. E coli that can`t express colicin-N immunity protein would be killed by colicin-N, colicin-N immunity protein is used to protect itself from attack of extracellular colicin-N and lysis protein helps the release of colicin-N. This part is constructed in the aim of achieve our “aggressive” design.

Ientification

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 125
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 65
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 696
Illegal AgeI site found at 1700
1000
COMPATIBLE WITH RFC[1000]

Design Notes

Each CDS are added an RBS (BBa_B0034) behind, and contain a terminator(BBa_B0010)

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 ===Summary===
-This is a composite part consist of an arabinose promoter (<partinfo>BBa_K206000</partinfo>), CDS of colicin-N (<partinfo>BBa_K2922027</partinfo>), CDS of colicin-N immunity protein (<partinfo>BBa_K2922028</partinfo>) and CDS of lysis protein (<partinfo>BBa_K2922029</partinfo>). Each CDS has an RBS (<partinfo>BBa_B0034</partinfo>) behind. Arabinose promoter could be induced by Arabinose in Escherichia coli BL21 strain and then express all proteins mentioned. E coli that can`t express colicin-N immunity protein would be killed by colicin-N, colicin-N immunity protein is used to protect itself from attack of extracellular colicin-N and kil protein helps the release of colicin-N. This part is constructed in the aim of achieve our “aggressive” design.
+This is a composite part consist of an arabinose promoter (<partinfo>BBa_K206000</partinfo>), CDS of colicin-N (<partinfo>BBa_K2922027</partinfo>), CDS of colicin-N immunity protein (<partinfo>BBa_K2922028</partinfo>) and CDS of lysis protein (<partinfo>BBa_K2922029</partinfo>). Each CDS has an RBS (<partinfo>BBa_B0034</partinfo>) behind. Arabinose promoter could be induced by Arabinose in Escherichia coli BL21 strain and then express all proteins mentioned. E coli that can`t express colicin-N immunity protein would be killed by colicin-N, colicin-N immunity protein is used to protect itself from attack of extracellular colicin-N and lysis protein helps the release of colicin-N. This part is constructed in the aim of achieve our “aggressive” design.
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