Difference between revisions of "Part:BBa K3187044"

Revision as of 08:12, 20 October 2019

P22 Bacteriophag Coat Protein (T7 promotor)

This part is a generator for the P22 bacteriophage coat protein fused to a LPETGG-tag for Sortase-mediated ligation of GGGG-tagged proteins. This part was used together with <a

                   href=" https://parts.igem.org/Part:BBa_K3187043" target="_blank">BBa_K3187043</a> for the dual expression of the P22 scaffold protein and the P22 coat protein to generated P22 Virus-like particles, that can be modified on their surface using sortase-mediated ligation. The potential of fine tuning the expression levels of both parts was tested via <a
                   href=" https://parts.igem.org/Part:BBa_K3187011" target="_blank">BBa_K3187011</a>.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal XbaI site found at 1499
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 98
23
INCOMPATIBLE WITH RFC[23]
Illegal XbaI site found at 1499
25
INCOMPATIBLE WITH RFC[25]
Illegal XbaI site found at 1499
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3187044 short</partinfo>
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+This part is a generator for the P22 bacteriophage coat protein fused to a LPETGG-tag for Sortase-mediated ligation of GGGG-tagged proteins. This part was used together with <a
+                    href=" https://parts.igem.org/Part:BBa_K3187043" target="_blank">BBa_K3187043</a> for the dual expression of the P22 scaffold protein and the P22 coat protein to generated P22 Virus-like particles, that can be modified on their surface using sortase-mediated ligation. The potential of fine tuning the expression levels of both parts was tested via <a
+                    href=" https://parts.igem.org/Part:BBa_K3187011" target="_blank">BBa_K3187011</a>.
 <!-- Add more about the biology of this part here