Difference between revisions of "Part:BBa K2610042"

Latest revision as of 01:57, 18 October 2018

pGapA-spisPink

Promoter GapA is naturally occurring constitutive promoter in Escherichia coli .

Usage and Biology

We, iGEM Leiden 2018, have combined this naturally occurring constitutive promoter ([BBa_K1033932) from Escherichia coli with the pink chromoprotein spisPink ([BBa_K592009) in order for us to compare it to our novel stress promoter+chromoprotein constructs.

Contrary to our stress promoters, this promoter has a constitutive high basal expression level. In contrast to many synthetic promoters, this novel promoter provides an indication of high levels of natural expression. Therefore, this promoter can be used as a future positive control of reporter production - such as GFP - in a natural situation. Moreover, GapA being a natural occurring promoter means that there are still possibilities for optimization through radiation. This way variations of this promoter can provide access to an even wider spectrum of colors.

Figure 1. Constitutively expressed pGapA-AmilCP compared to non-transformed DH5a and pGapA-spis-Pink (pink chromoprotein)

References

Munjal, N., Jawed, K., Wajid, S., & Yazdani, S. S. (2015). A constitutive expression system for cellulase secretion in Escherichia coli and its use in bioethanol production. PloS one, 10(3), e0119917.

Sequence and Features