Difference between revisions of "Part:BBa K2684006"
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CsgA fused with SpyTag by 2xGGGGS linker | CsgA fused with SpyTag by 2xGGGGS linker | ||
− | This part was improved from | + | <br> |
+ | This part was improved from BBa_K1583000: https://parts.igem.org/Part:BBa_K1583000 | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
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− | <h2> | + | <h2>Usage and Biology</h2> |
<p> | <p> | ||
We added a SpyTag sequence which fused after <i>csgA</i> gene, creating <i>csgA-spytag</i> (BBa_K2684006). With SpyTag, CotA laccase can be fixed onto the biofilm by forming a covalent bond SpyTag-SpyCatcher.</p> | We added a SpyTag sequence which fused after <i>csgA</i> gene, creating <i>csgA-spytag</i> (BBa_K2684006). With SpyTag, CotA laccase can be fixed onto the biofilm by forming a covalent bond SpyTag-SpyCatcher.</p> |
Revision as of 21:28, 17 October 2018
CsgA-SpyTag
CsgA fused with SpyTag by 2xGGGGS linker
This part was improved from BBa_K1583000: https://parts.igem.org/Part:BBa_K1583000
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Usage and Biology
We added a SpyTag sequence which fused after csgA gene, creating csgA-spytag (BBa_K2684006). With SpyTag, CotA laccase can be fixed onto the biofilm by forming a covalent bond SpyTag-SpyCatcher.