Difference between revisions of "Part:BBa K2656009"
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BBa_K2656008 is the RBS [https://parts.igem.org/Part:BBa_K2656009 BBa_K2656009] standardized into the Golden Braid assembly method. Thus, it is strong ribosome binding site compatible both with the BioBrick and Golden Gate grammar.  
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BBa_K2656009 is the original [https://parts.igem.org/Part:BBa_K2656102 BBa_K2656102 BBa_B0030] RBS standardized into the Golden Braid assembly method. Thus, it is strong ribosome binding site compatible both with the BioBrick and Golden Gate grammar.  
  
 
Using the [http://2018.igem.org/Team:Valencia_UPV/Protocols Golden Gate assembly protocol], it can be combined with other GB adapted basic parts, such as the ones from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Valencia UPV IGEM 2018 Printeria Collection], to assemble transcriptional units in a single one-pot reaction.  
 
Using the [http://2018.igem.org/Team:Valencia_UPV/Protocols Golden Gate assembly protocol], it can be combined with other GB adapted basic parts, such as the ones from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Valencia UPV IGEM 2018 Printeria Collection], to assemble transcriptional units in a single one-pot reaction.  
  
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Characterization of the this part was performed with the transcriptional unit [https://parts.igem.org/Part:BBa_K2656101 BBa_K2656101 ], which was used in a comparative RBS expression experiment with composite parts [https://parts.igem.org/Part:BBa_K2656102 BBa_K2656102 ] and [https://parts.igem.org/Part:BBa_K2656103 BBa_K2656103].  
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Characterization of the this part was performed with the transcriptional unit [https://parts.igem.org/Part:BBa_K2656101 BBa_K2656101], which was used in a comparative RBS expression experiment with composite parts [https://parts.igem.org/Part:BBa_K2656102 BBa_K2656102 ] and [https://parts.igem.org/Part:BBa_K2656103 BBa_K2656103].  
 
They all were assembled in a Golden Braid alpha1 plasmid using the same promoter, CDS and terminator:
 
They all were assembled in a Golden Braid alpha1 plasmid using the same promoter, CDS and terminator:
 
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Revision as of 16:05, 12 October 2018

BBa_K2656009 is the original BBa_K2656102 BBa_B0030 RBS standardized into the Golden Braid assembly method. Thus, it is strong ribosome binding site compatible both with the BioBrick and Golden Gate grammar.

Using the [http://2018.igem.org/Team:Valencia_UPV/Protocols Golden Gate assembly protocol], it can be combined with other GB adapted basic parts, such as the ones from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Valencia UPV IGEM 2018 Printeria Collection], to assemble transcriptional units in a single one-pot reaction.

Characterization of the this part was performed with the transcriptional unit BBa_K2656101, which was used in a comparative RBS expression experiment with composite parts BBa_K2656102 and BBa_K2656103. They all were assembled in a Golden Braid alpha1 plasmid using the same promoter, CDS and terminator:

  • Promoter BBa_K2656004: the J23106 promoter in its Golden Braid compatible version from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Part Collection]
  • CDS BBa_K2656013: the BBa_K2656009 sfGFP sequence in its Golden Braid standardized version from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Part Collection]
  • Terminator BBa_K2656026: the B0015 transcriptional terminator in its Golden Braid compatible version from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Part Collection]


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]