Difference between revisions of "Part:BBa K2656008"
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<partinfo>BBa_K2656008 short</partinfo>
 
<partinfo>BBa_K2656008 short</partinfo>
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Part BBa_K2656008 is the RBS [https://parts.igem.org/Part:BBa_J61100 BBa_J61100] standardized into the Golden Braid assembly method. Thus, it is a very weak RBS compatible both with the BioBrick and Golden Gate grammar.  
 
Part BBa_K2656008 is the RBS [https://parts.igem.org/Part:BBa_J61100 BBa_J61100] standardized into the Golden Braid assembly method. Thus, it is a very weak RBS compatible both with the BioBrick and Golden Gate grammar.  
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Using the Golden Gate assembly protocol [http://2018.igem.org/Team:Valencia_UPV/Protocols Golden Gate assembly protocol], it can be combined with other Golden Braid compatible basic parts from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Valencia UPV IGEM 2018 Printeria Collection] to assemble transcriptional units in a single one-pot reaction.  
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Using the Golden Gate assembly protocol [http://2018.igem.org/Team:Valencia_UPV/Protocols Golden Gate assembly protocol], it can be combined with other basic parts from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Valencia UPV IGEM 2018 Printeria Collection] to assemble transcriptional units in a single one-pot reaction.  
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Characterization of this RBS was performed with the transcriptional unit [https://parts.igem.org/Part:BBa_K2656100 BBa_K2656100], which was used in an RBS expression experiment with composite parts [https://parts.igem.org/Part:BBa_K2656104 BBa_K2656104] and [https://parts.igem.org/Part:BBa_K2656101 BBa_K2656101]. They all were assembled in a Golden Braid alpha1 plasmid using the same promoter, CDS and terminator:
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Promoter BBa_K2656004: BBa_J23106 in its Golden Braid compatible version
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CDS BBa_K2656013: sfGFP BBa_I746916 in its Golden Braid compatible version 
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Terminator BBa_K2656026: BBa_B0015 Golden Braid standardized version
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Revision as of 15:18, 12 October 2018


Very Weak Ribosome Binding Site J61100

Part BBa_K2656008 is the RBS BBa_J61100 standardized into the Golden Braid assembly method. Thus, it is a very weak RBS compatible both with the BioBrick and Golden Gate grammar.

Using the Golden Gate assembly protocol [http://2018.igem.org/Team:Valencia_UPV/Protocols Golden Gate assembly protocol], it can be combined with other basic parts from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Valencia UPV IGEM 2018 Printeria Collection] to assemble transcriptional units in a single one-pot reaction.

Characterization of this RBS was performed with the transcriptional unit BBa_K2656100, which was used in an RBS expression experiment with composite parts BBa_K2656104 and BBa_K2656101. They all were assembled in a Golden Braid alpha1 plasmid using the same promoter, CDS and terminator: Promoter BBa_K2656004: BBa_J23106 in its Golden Braid compatible version CDS BBa_K2656013: sfGFP BBa_I746916 in its Golden Braid compatible version Terminator BBa_K2656026: BBa_B0015 Golden Braid standardized version


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]