Difference between revisions of "Part:BBa J45700"
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We assembled a complete wintergreen odor biosynthetic system (<partinfo>BBa_J45700</partinfo>) by combining the salicylic acid (<partinfo>BBa_J45320</partinfo>) and wintergreen odor generators (<partinfo>BBa_J45120</partinfo>). <partinfo>BBa_J45700</partinfo> catalyzes the conversion of the cellular metabolite chorismate to methyl salicylate or wintergreen odor. | We assembled a complete wintergreen odor biosynthetic system (<partinfo>BBa_J45700</partinfo>) by combining the salicylic acid (<partinfo>BBa_J45320</partinfo>) and wintergreen odor generators (<partinfo>BBa_J45120</partinfo>). <partinfo>BBa_J45700</partinfo> catalyzes the conversion of the cellular metabolite chorismate to methyl salicylate or wintergreen odor. | ||
| − | [[Image:BBa_J45700|center]] | + | [[Image:BBa_J45700.png|center]] |
===Usage and Biology=== | ===Usage and Biology=== | ||
Latest revision as of 23:47, 8 March 2008
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We assembled a complete wintergreen odor biosynthetic system (Status: 500
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) by combining the salicylic acid (Status: 500 Content-type: text/html
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) and wintergreen odor generators (Status: 500 Content-type: text/html
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). Status: 500 Content-type: text/html
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catalyzes the conversion of the cellular metabolite chorismate to methyl salicylate or wintergreen odor.
Usage and Biology
To construct a complete wintergreen odor biosynthetic system, we needed to design an additional biosynthetic device for wintergreen odor precursor production. We designed a biosynthetic device for production of salicylate from the cellular metabolite chorismate (Status: 500 Content-type: text/html
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) based on the pchBA genes from Pseudomonas aeruginosa. The enzyme PchA, an isochorismate synthase, catalyzes the conversion of chorismate to isochorismate. The enzyme PchB, an isochorismate pyruvate-lyase, catalyzes the conversion of isochorismate to salicylate and pyruvate. Serino et al. previously demonstrated salicylate formation in E. coli using the pchBA genes.
Sequence and Features Status: 500 Content-type: text/html
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