Difference between revisions of "Part:BBa J45199:Experience"
| Line 32: | Line 32: | ||
==Characterization== | ==Characterization== | ||
| − | === | + | ===Growth dependent banana odor production=== |
The ATF1 enzyme generator (<partinfo>BBa_J45199</partinfo>) is used in both banana odor biosynthetic devices (<partinfo>BBa_J45200</partinfo> and <partinfo>BBa_J45250</partinfo>). | The ATF1 enzyme generator (<partinfo>BBa_J45199</partinfo>) is used in both banana odor biosynthetic devices (<partinfo>BBa_J45200</partinfo> and <partinfo>BBa_J45250</partinfo>). | ||
| Line 39: | Line 39: | ||
<br style="clear:both;"/> | <br style="clear:both;"/> | ||
| − | ===Smell test of banana odor generator <partinfo>BBa_J45200</partinfo> | + | ===Smell test of banana odor generator=== |
| + | The banana odor generator (<partinfo>BBa_J45200</partinfo>) includes the ATF1 enzyme generator (<partinfo>BBa_J45199</partinfo>) under the control of a constitutive promoter (<partinfo>BBa_R0040</partinfo>). | ||
{{:IGEM:MIT/2006/Smell test}} | {{:IGEM:MIT/2006/Smell test}} | ||
<br style="clear:both;"/> | <br style="clear:both;"/> | ||
Revision as of 02:22, 8 March 2008
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_J45199
Banana odor based reporter of transcriptional signal.
User Reviews
UNIQ68e17a9f60f8a40d-partinfo-00000000-QINU
|
••••• |
BBa_J45199 was successfully used to produce banana odor under the control of BBa_R0040 and BBa_J45992in the banana odor generators BBa_J45200 and BBa_J45250, respectively. |
UNIQ68e17a9f60f8a40d-partinfo-00000007-QINU
Characterization
Growth dependent banana odor production
The ATF1 enzyme generator (BBa_J45199) is used in both banana odor biosynthetic devices (BBa_J45200 and BBa_J45250).
Growth dependent banana odor production
To demonstrate growth phase dependent banana odor production, we compared the behavior of constitutive and stationary phase dependent banana odor generators (A and B, respectively). We measured isoamyl acetate concentrations of cultures of the constitutive and stationary phase banana odor generators at different culture cell densities (OD600nm) (C). As expected, the stationary phase banana odor generator produced very little isoamyl acetate at low cell densities but its isoamyl acetate production increased with cell density. By comparison, the constitutive banana odor generator produced more isoamyl acetate at lower cell densities than the stationary phase banana odor generator. To visually aid comparison of the two odor generators, an empirical fit to the data for each device is shown.
To demonstrate growth phase dependent banana odor production, we compared the behavior of constitutive and stationary phase dependent banana odor generators (A and B, respectively). We measured isoamyl acetate concentrations of cultures of the constitutive and stationary phase banana odor generators at different culture cell densities (OD600nm) (C). As expected, the stationary phase banana odor generator produced very little isoamyl acetate at low cell densities but its isoamyl acetate production increased with cell density. By comparison, the constitutive banana odor generator produced more isoamyl acetate at lower cell densities than the stationary phase banana odor generator. To visually aid comparison of the two odor generators, an empirical fit to the data for each device is shown.
Smell test of banana odor generator
The banana odor generator (BBa_J45200) includes the ATF1 enzyme generator (BBa_J45199) under the control of a constitutive promoter (BBa_R0040).
Smell tests of cultures of wintergreen and banana odor generators (BBa_J45120 and BBa_J45200, respectively)
At the 2006 iGEM Jamboree, we conducted blind smell tests in which iGEM participants had to distinguish between cell cultures producing wintergreen odor, banana odor and the natural fecal odor of E. coli. Of the 116 respondents, 64% were able to correctly identify the culture producing methyl salicylate through its wintergreen bouquet, 87% were able to correctly identify the culture producing isoamyl acetate through its banana bouquet, and 86% were able to correctly identify the laboratory E. coli strain TOP10 through its stink. The error bars are the specific margin of error at 95% confidence. For the smell tests, we used the odor-free chassis (E. coli strain YYC912) for the wintergreen and banana odor generators.
At the 2006 iGEM Jamboree, we conducted blind smell tests in which iGEM participants had to distinguish between cell cultures producing wintergreen odor, banana odor and the natural fecal odor of E. coli. Of the 116 respondents, 64% were able to correctly identify the culture producing methyl salicylate through its wintergreen bouquet, 87% were able to correctly identify the culture producing isoamyl acetate through its banana bouquet, and 86% were able to correctly identify the laboratory E. coli strain TOP10 through its stink. The error bars are the specific margin of error at 95% confidence. For the smell tests, we used the odor-free chassis (E. coli strain YYC912) for the wintergreen and banana odor generators.