Difference between revisions of "Part:BBa J45992:Design"
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Revision as of 23:39, 7 March 2008
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Status: 500
Content-type: text/html
Software error:
Global symbol "$groups" requires explicit package name at /websites/parts.igem.org/cgi/lib/User.pm line 84. Global symbol "$groups" requires explicit package name at /websites/parts.igem.org/cgi/lib/User.pm line 84. Global symbol "$groups" requires explicit package name at /websites/parts.igem.org/cgi/lib/User.pm line 85. Compilation failed in require at /websites/parts.igem.org/cgi/lib/IconBar.pm line 12. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/IconBar.pm line 12. Compilation failed in require at /websites/parts.igem.org/cgi/lib/BBWeb.pm line 9. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/BBWeb.pm line 9. Compilation failed in require at /websites/parts.igem.org/cgi/lib/Change.pm line 8. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Change.pm line 8. Compilation failed in require at /websites/parts.igem.org/cgi/lib/Part.pm line 12. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Part.pm line 12. Compilation failed in require at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8.
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Design Notes
- Constructed by PCR
Template
Escherichia coli strain MG1655
Forward Primer
5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GCT GGC ACA GGA ACG TTA TC - 3'
Reverse Primer
5'-GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TGT TAA ATA TAG ATC ACA ATT TTG AAA CCG- 3'
Source
- Promoter controlling expression of osmY from Escherichia coli (GenBank [http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=453110 S67278])
References
<biblio>
- Yim-1994 pmid=8282684
- Schellhorn-1998 pmid=9829938
- Wise pmid=8631665
- Funabashi pmid=11879714
- Makinoshima pmid=11985708
- Yim-1992 pmid=1317380
- Hengge-Aronis-1993 pmid=8416901
- Vijaykumar-2004 pmid=15576800
</biblio>
Neidhart, Frederick C. Escherichia coli and Salmonella. Washington: ASM Press, 1996.