Difference between revisions of "Part:BBa J45992"
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<partinfo>BBa_J45992 short</partinfo> | <partinfo>BBa_J45992 short</partinfo> | ||
| − | <partinfo>BBa_J45992</partinfo> is a stationary phase promoter derived from the promoter that controls transcription of ''osmY'' in ''Escherichia coli'' <cite>Yim-1992, Yim-1994<cite>. <partinfo>BBa_J45992</partinfo> is active in stationary phase and under high osmotic pressure conditions. | + | <partinfo>BBa_J45992</partinfo> is a stationary phase promoter derived from the promoter that controls transcription of ''osmY'' in ''Escherichia coli'' <cite>Yim-1992, Yim-1994</cite>. <partinfo>BBa_J45992</partinfo> is active in stationary phase and under high osmotic pressure conditions. |
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 23:27, 7 March 2008
Full-length stationary phase osmY promoter
BBa_J45992 is a stationary phase promoter derived from the promoter that controls transcription of osmY in Escherichia coli Yim-1992, Yim-1994. BBa_J45992 is active in stationary phase and under high osmotic pressure conditions.
Usage and Biology
Expression of osmY is dependent on σS in vivo Hengge-Aronis-1993. Schellhorn et al. previously demonstrated that an osmY::lacZ fusion generated the highest transcriptional signal in stationary phase as compared to nine other σS-dependent promoter-lacZ fusions Schellhorn-1998, Vijayakumar-2004. In addition, the osmY::lacZ fusion generated only a small transcriptional signal during exponential growth.
<biblio>
- Yim-1994 pmid=8282684
- Yim-1992 pmid=1317380
- Hengge-Aronis-1993 pmid=8416901
- Vijaykumar-2004 pmid=15576800
- Schellhorn-1998 pmid=9829938
</biblio>
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]