Difference between revisions of "Part:BBa K2842680"
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K2842680 short</partinfo>
 
<partinfo>BBa_K2842680 short</partinfo>
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 +
{| style="color:black" cellpadding="6" cellspacing="1" border="2" align="right"
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! colspan="2" style="background:#FFBF00;"|Intein Monomer 1
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|-
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|'''Function'''
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|Standardised blue-white screening
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|-
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|'''Use in'''
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|E. coli cells
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|-
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|'''Chassis Tested'''
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|DH5α cells, BL21* cells
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|-
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|'''Abstraction Hierarchy'''
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|Composite Device
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|-
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|'''Related Device'''
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|[https://parts.igem.org/Part:BBa_K1362101 BBa_K1362101]
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|-
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|'''RFC standard'''
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|[https://parts.igem.org/Help:Assembly_standard_10 RFC10],[https://parts.igem.org/Help:Assembly_standard_21 RFC21],[https://parts.igem.org/Help:Assembly_standard_23 RFC23] <br> & [https://parts.igem.org/Help:Assembly_standard_25 RFC25] compatible
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|-
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|'''Backbone'''
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|pSB1C3<br>
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|-
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|'''Submitted by'''
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|[http://2018.igem.org/Team:UCL UCL iGEM 2018]
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|}
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 +
  
  

Revision as of 16:49, 17 October 2018


Intein Monomer 1: RFP reporter flanked with orthogonal inteins

Intein Monomer 1
Function Standardised blue-white screening
Use in E. coli cells
Chassis Tested DH5α cells, BL21* cells
Abstraction Hierarchy Composite Device
Related Device BBa_K1362101
RFC standard RFC10,RFC21,RFC23
& RFC25 compatible
Backbone pSB1C3
Submitted by [http://2018.igem.org/Team:UCL UCL iGEM 2018]



Figure 1: BsaI digestions

(1) BsaI digested Intein Passenger BBa_K2842669
(2) BsaI digested RFP inteins BBa_K2842680
(3) BsaI digested GFP inteins BBa_K2842690
*edited to show relevant bands





















-

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1165
    Illegal BsaI.rc site found at 28
    Illegal SapI site found at 903
    Illegal SapI.rc site found at 213