Difference between revisions of "Part:BBa J45993:Design"
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<partinfo>BBa_J45993 short</partinfo> | <partinfo>BBa_J45993 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
| − | ==Forward Primer== | + | ====Forward Primer==== |
5'-CTA GAG GCT TAT GTT TTC GCT GAT ATC CCG AGC GGT TTC AAA ATT GTG ATC TAT ATT TAA CAA ATA CTA GTA GCG GCC GCT GCA-3' | 5'-CTA GAG GCT TAT GTT TTC GCT GAT ATC CCG AGC GGT TTC AAA ATT GTG ATC TAT ATT TAA CAA ATA CTA GTA GCG GCC GCT GCA-3' | ||
| − | ==Reverse Primer== | + | ====Reverse Primer==== |
5'-GCG GCC GCT ACT AGT ATT TGT TAA ATA TAG ATC ACA ATT TTG AAA CCG CTC GGG ATA TCA GCG AAA ACA TAA GCC T-3' | 5'-GCG GCC GCT ACT AGT ATT TGT TAA ATA TAG ATC ACA ATT TTG AAA CCG CTC GGG ATA TCA GCG AAA ACA TAA GCC T-3' | ||
Revision as of 15:53, 5 February 2008
Minimal stationary phase osmY promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Forward Primer
5'-CTA GAG GCT TAT GTT TTC GCT GAT ATC CCG AGC GGT TTC AAA ATT GTG ATC TAT ATT TAA CAA ATA CTA GTA GCG GCC GCT GCA-3'
Reverse Primer
5'-GCG GCC GCT ACT AGT ATT TGT TAA ATA TAG ATC ACA ATT TTG AAA CCG CTC GGG ATA TCA GCG AAA ACA TAA GCC T-3'
Source
This part was constructed from the annealing of the primers listed in the Design Notes section.
References
<biblio>
- Yim pmid=8282684
- Schellhorn pmid=9829938
- Wise pmid=8631665
- Funabashi pmid=11879714
- Makinoshima pmid=11985708
</biblio>
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=453110 S67278]
Neidhart, Frederick C. Escherichia coli and Salmonella. Washington: ASM Press, 1996.