Difference between revisions of "Part:BBa J45992:Design"
(→Forward Primer) |
(→References) |
||
| Line 21: | Line 21: | ||
===References=== | ===References=== | ||
<biblio> | <biblio> | ||
| − | #Yim pmid=8282684 | + | #Yim-1994 pmid=8282684 |
| − | #Schellhorn pmid=9829938 | + | #Schellhorn-1998 pmid=9829938 |
#Wise pmid=8631665 | #Wise pmid=8631665 | ||
#Funabashi pmid=11879714 | #Funabashi pmid=11879714 | ||
#Makinoshima pmid=11985708 | #Makinoshima pmid=11985708 | ||
| + | #Yim-1992 pmid=1317380 | ||
| + | #Hengge-Aronis-1993 pmid=8416901 | ||
| + | #Vijaykumar-2004 pmid=15576800 | ||
</biblio> | </biblio> | ||
Revision as of 23:27, 7 March 2008
Status: 500
Content-type: text/html
Software error:
Global symbol "$groups" requires explicit package name at /websites/parts.igem.org/cgi/lib/User.pm line 84. Global symbol "$groups" requires explicit package name at /websites/parts.igem.org/cgi/lib/User.pm line 84. Global symbol "$groups" requires explicit package name at /websites/parts.igem.org/cgi/lib/User.pm line 85. Compilation failed in require at /websites/parts.igem.org/cgi/lib/IconBar.pm line 12. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/IconBar.pm line 12. Compilation failed in require at /websites/parts.igem.org/cgi/lib/BBWeb.pm line 9. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/BBWeb.pm line 9. Compilation failed in require at /websites/parts.igem.org/cgi/lib/Change.pm line 8. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Change.pm line 8. Compilation failed in require at /websites/parts.igem.org/cgi/lib/Part.pm line 12. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Part.pm line 12. Compilation failed in require at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8.
For help, please send mail to this site's webmaster, giving this error message and the time and date of the error.
Status: 500
Content-type: text/html
Software error:
Global symbol "$groups" requires explicit package name at /websites/parts.igem.org/cgi/lib/User.pm line 84. Global symbol "$groups" requires explicit package name at /websites/parts.igem.org/cgi/lib/User.pm line 84. Global symbol "$groups" requires explicit package name at /websites/parts.igem.org/cgi/lib/User.pm line 85. Compilation failed in require at /websites/parts.igem.org/cgi/lib/IconBar.pm line 12. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/IconBar.pm line 12. Compilation failed in require at /websites/parts.igem.org/cgi/lib/BBWeb.pm line 9. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/BBWeb.pm line 9. Compilation failed in require at /websites/parts.igem.org/cgi/lib/Change.pm line 8. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Change.pm line 8. Compilation failed in require at /websites/parts.igem.org/cgi/lib/Part.pm line 12. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Part.pm line 12. Compilation failed in require at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8.
For help, please send mail to this site's webmaster, giving this error message and the time and date of the error.
Design Notes
Primers used to PCR this part out of the E.coli genome:
Forward Primer
5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GCT GGC ACA GGA ACG TTA TC - 3'
Reverse Primer
5'-GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TGT TAA ATA TAG ATC ACA ATT TTG AAA CCG- 3'
Source
This part was PCRed out of E. coli's genome with the primers listed in the design notes section.
References
<biblio>
- Yim-1994 pmid=8282684
- Schellhorn-1998 pmid=9829938
- Wise pmid=8631665
- Funabashi pmid=11879714
- Makinoshima pmid=11985708
- Yim-1992 pmid=1317380
- Hengge-Aronis-1993 pmid=8416901
- Vijaykumar-2004 pmid=15576800
</biblio>
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=453110 S67278]
Neidhart, Frederick C. Escherichia coli and Salmonella. Washington: ASM Press, 1996.