Difference between revisions of "Part:BBa J45005:Design"

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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_J45005 short</partinfo>
 
<partinfo>BBa_J45005 short</partinfo>
  
 
<partinfo>BBa_J45005 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_J45005 SequenceAndFeatures</partinfo>
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===Design Notes===
 
===Design Notes===
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#no restriction enzyme cut sites in coding region
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#No BioBrick restriction enzyme cut sites in coding region
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#changed natural stop codon to TAA TAA
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#Changed natural stop codon to TAA TAA
 +
#No template DNA available and thus could not construct by PCR
  
 
===Source===
 
===Source===
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#GenBank Accession No. [http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=6002711 AF133053]
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''SAMT'' from ''Clarkia breweri'' ([http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=6002711 GenBank AF133053])
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#Species: ''Clarkia breweri''
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===References===
 
===References===
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#Ross-ArchBiochemBiophys-1999 pmid=10375393
 
#Ross-ArchBiochemBiophys-1999 pmid=10375393
 
</biblio>
 
</biblio>
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*Wiki Link: http://openwetware.org/wiki/IGEM:MIT/2006/Notebook/2006-6-6
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*[http://openwetware.org/wiki/IGEM:MIT/2006/Notebook/2006-6-6 MIT iGEM 2006 notebook entry]

Revision as of 22:01, 9 March 2008

SAM:salicylic acid carboxyl methyltransferase; converts salicylic acid to methyl salicylate (winterg


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 652
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 232

Design Notes

  1. No BioBrick restriction enzyme cut sites in coding region
  2. Changed natural stop codon to TAA TAA
  3. No template DNA available and thus could not construct by PCR

Source

SAMT from Clarkia breweri ([http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=6002711 GenBank AF133053])

References

<biblio>

  1. Ross-ArchBiochemBiophys-1999 pmid=10375393

</biblio>

  • [http://openwetware.org/wiki/IGEM:MIT/2006/Notebook/2006-6-6 MIT iGEM 2006 notebook entry]