Difference between revisions of "Part:BBa J45999"

Revision as of 19:49, 9 March 2008

E. coli strain YYC912; Odor-free chassis

Escherichia coli strain [http://cgsc2.biology.yale.edu/Strain.php?ID=64826 YYC912]. Biosafety level 1 organism derived from Escherichia coli K12.

BBa_J45999 has a mutation in the tnaA gene which encodes a tryptophanase which converts tryptophan to indole. Indole is responsible for the foul stench of E. coli so tnaA mutants can have reduced indole levels and thus reduced foul odor.

Usage and Biology

CGSC#: 7602
Strain Designation
- Designation: YYC912
- Source Person: Cronan & Chang
- Choice: 1
Sex(Hfr,F+,F-,or F'): F-
Mutations
- DE(argF-lac)169
- LAM-
- poxB15::lacZ::CmR Note chloramphenicol resistantance
- IN(rrnD-rrnE)1
- rph-1
- tnaA5
No. of Muts Carried: 6
Special Uses: lac fusion-stat.phase promoter
Has_Partner_Isogenic_WRT: poxB15::lacZ::CmR
Isogenic_Partner_Is: ZK126
References
- Chang, Y.-Y., A.Y. Wang, J.E. Cronan 1994. Expression of Escherichia coli pyruvate oxidase (PoxB) depends on the sigma factor encoded by the rpoS(katF) gene. Mol.Microbiol. 11:1019-1028
ID#: 64826

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 7: / Line 7: @@
 ===Usage and Biology===
-This ''E. coli'' strain is chloramphenicol-resistant.
+* CGSC#: 7602
+* Strain Designation
+**Designation: YYC912
+**Source Person: Cronan & Chang
+**Choice: 1
+* Sex(Hfr,F+,F-,or F'): F-
+* Mutations
+**DE(argF-lac)169
+**LAM-
+**poxB15::lacZ::CmR  '''Note chloramphenicol resistantance'''
+**IN(rrnD-rrnE)1
+**rph-1
+**tnaA5
+* No. of Muts Carried: 6
+* Special Uses: lac fusion-stat.phase promoter
+* Has_Partner_Isogenic_WRT: poxB15::lacZ::CmR
+* Isogenic_Partner_Is: ZK126
+* References
+**Chang, Y.-Y., A.Y. Wang, J.E. Cronan 1994. Expression of Escherichia coli pyruvate oxidase (PoxB) depends on the sigma factor encoded by the rpoS(katF) gene. Mol.Microbiol. 11:1019-1028
+* ID#: 64826
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