Difference between revisions of "Part:BBa J45009"
Line 2: Line 2:
 
<partinfo>BBa_J45009 short</partinfo>
 
<partinfo>BBa_J45009 short</partinfo>
  
−
*'''Very important note: None of the indicated base changes were made.  Please consult design page to determine how we worked with this part.'''
+
<partinfo>BBa_J45009</partinfo> encodes a branched-chain amino acid transaminase derived from the ''THI3'' gene from ''Saccharomyces cerevisiae''.  THI3 catalyzes the second step in isoamyl alcohol biosynthesis: conversion of &alpha;-ketoisocaproate to 3-methylbutanal via enzymatic reaction 4.1.1.1.
  
−
This part catalyzes the second step in isoamyl alcohol biosynthesis.  It converts alpha-ketoisocaproate to 3-methylbutanal via enzymatic reaction 4.1.1.1.  3-methylbutanal is then reduced via a alcohol dehydrogenase to isoamyl alcohol (the precursor to isoamyl acetate or banana scent).
 
−
 
−
<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
−
THI3 is formerly known as KID1.
+
*''THI3'' is formerly known as ''KID1''.
 +
*3-methylbutanal is reduced via a chassis alcohol dehydrogenase to isoamyl alcohol which is a precursor to isoamyl acetate (banana odor).
 +
 
 +
*'''Note: <partinfo>BBa_J45009</partinfo> has several internal BioBrick restriction sites.  We indicate proposed silent mutations for eliminating the BioBrick restriction sites.  Please consult design page to determine how we worked with this part.'''
  
 
<!-- -->
 
<!-- -->

Revision as of 21:36, 7 March 2008

ketoisocaproate decarboxylase (THI3); converts alpha-ketoisocaproate to 3-methylbutanal

BBa_J45009 encodes a branched-chain amino acid transaminase derived from the THI3 gene from Saccharomyces cerevisiae. THI3 catalyzes the second step in isoamyl alcohol biosynthesis: conversion of α-ketoisocaproate to 3-methylbutanal via enzymatic reaction 4.1.1.1.

Usage and Biology

  • THI3 is formerly known as KID1.
  • 3-methylbutanal is reduced via a chassis alcohol dehydrogenase to isoamyl alcohol which is a precursor to isoamyl acetate (banana odor).
  • Note: BBa_J45009 has several internal BioBrick restriction sites. We indicate proposed silent mutations for eliminating the BioBrick restriction sites. Please consult design page to determine how we worked with this part.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3
    Illegal EcoRI site found at 904
    Illegal EcoRI site found at 1603
    Illegal EcoRI site found at 1789
    Illegal SpeI site found at 697
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3
    Illegal EcoRI site found at 904
    Illegal EcoRI site found at 1603
    Illegal EcoRI site found at 1789
    Illegal SpeI site found at 697
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3
    Illegal EcoRI site found at 904
    Illegal EcoRI site found at 1603
    Illegal EcoRI site found at 1789
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3
    Illegal EcoRI site found at 904
    Illegal EcoRI site found at 1603
    Illegal EcoRI site found at 1789
    Illegal SpeI site found at 697
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3
    Illegal EcoRI site found at 904
    Illegal EcoRI site found at 1603
    Illegal EcoRI site found at 1789
    Illegal SpeI site found at 697
  • 1000
    COMPATIBLE WITH RFC[1000]