Difference between revisions of "Part:BBa K2835000:Design"
| Line 15: | Line 15: | ||
===Source=== | ===Source=== | ||
| − | GenBank: AF506027.1 | + | Part is originally derived from GenBank: AF506027.1 <br> |
| − | https://www.ncbi.nlm.nih.gov/nuccore?cmd=Retrieve&db=nucleotide&dopt=GenBank&list_uids=21464837 | + | |
| + | https://www.ncbi.nlm.nih.gov/nuccore?cmd=Retrieve&db=nucleotide&dopt=GenBank&list_uids=21464837 <br> | ||
from Discosoma striata (coral) | from Discosoma striata (coral) | ||
===References=== | ===References=== | ||
Revision as of 13:25, 30 September 2018
Enigneered mutant of mRFP1 + RBS (compatible with all iGEM RFC assembly standards)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part contains improved version of the BBa_E1010 red flourescent protein. To make the BioBrick compatible to assembly standard RFC[25], two silent mutations were made to remove two AgeI sites: The AgeI site (accggt) at 573 bp was changed to accagt. The AgeI site (accggt) at 685 bp was changed to acgggt.
Part is originally derived from GenBank: AF506027.1
Source
Part is originally derived from GenBank: AF506027.1
https://www.ncbi.nlm.nih.gov/nuccore?cmd=Retrieve&db=nucleotide&dopt=GenBank&list_uids=21464837
from Discosoma striata (coral)