Difference between revisions of "Part:BBa K2810011"
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<partinfo>BBa_K2810011 short</partinfo> | <partinfo>BBa_K2810011 short</partinfo> | ||
− | mCherry, codon optimised for <i>Nicotiana benthamiana</i>, under control of part [https://parts.igem.org/Part:BBa_J23100 BBa_J23100] (bacterial promoter) and [https://parts.igem.org/Part:BBa_B0015 BBa_B0015] (bacterial terminator). The RBS is part [https://parts.igem.org/Part:BBa_B0030 BBa_B0030] between the promoter and CDS. This part was used to show that the coding sequence of our mCherry part [https://parts.igem.org/wiki/index.php?title=Part:BBa_K2810009 (BBa_K2810009)] was fully functional. The plasmid it is in (pSB1C3) encodes a chloramphenicol resistance gene. | + | mCherry, codon optimised for <i>Nicotiana benthamiana</i>, under control of part [https://parts.igem.org/Part:BBa_J23100 BBa_J23100] (bacterial promoter) and [https://parts.igem.org/Part:BBa_B0015 BBa_B0015] (bacterial terminator). The RBS is part [https://parts.igem.org/Part:BBa_B0030 BBa_B0030] between the promoter and CDS. This part was used to show that the coding sequence of our mCherry part [https://parts.igem.org/wiki/index.php?title=Part:BBa_K2810009 (BBa_K2810009)] was fully functional. The plasmid it is in ([https://parts.igem.org/wiki/index.php/Part:pSB1C3 pSB1C3]) encodes a chloramphenicol resistance gene. |
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Latest revision as of 22:10, 26 September 2018
mCherry under control of the bacterial promoter and terminator
mCherry, codon optimised for Nicotiana benthamiana, under control of part BBa_J23100 (bacterial promoter) and BBa_B0015 (bacterial terminator). The RBS is part BBa_B0030 between the promoter and CDS. This part was used to show that the coding sequence of our mCherry part (BBa_K2810009) was fully functional. The plasmid it is in (pSB1C3) encodes a chloramphenicol resistance gene.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]