Difference between revisions of "Part:BBa K2810007"

Revision as of 10:41, 31 August 2018

siRNA from 5' region of Myzus persicae SP3 secreted protein

SP3 is a predicted secreted protein that localizes to [http://rspb.royalsocietypublishing.org/content/280/1750/20121952 aphids bacteriocyte cells].

This sequence is taken from the coding strand from a 5’ region of the Myzus persisae BCR3 gene. The Cardiff_Wales team is attempting to target this gene using RNAi.

SP3_5_5 is cloned into the golden gate version of pSB1C3 (P10500) using BsmBI or Esp3I and conforms to the [http://2016.igem.org/Resources/Plant_Synthetic_Biology/PhytoBricks Phytobrick standard].

It is flanked by BsaI sites together 5' TACT and 3' CCAT cloning sequences for subsequent cloning into a level 1 plasmid.

This sequence is incorporated into a level 1 plasmid for plant expression as part of an RNAi construct with the complimentary sequence found in BBa_K2810008.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 4: / Line 4: @@
 SP3 is a predicted secreted protein that localizes to [http://rspb.royalsocietypublishing.org/content/280/1750/20121952 aphids  bacteriocyte cells].
-This sequence is taken from the complement strand from a 5’ region of the Myzus persicae SP3 gene. The Cardiff_Wales team is attempting to target this gene using RNAi.
+This sequence is taken from the coding strand from a 5’ region of the Myzus persisae BCR3 gene. The Cardiff_Wales team is attempting to target this gene using RNAi.
-SP3_5_3 is cloned into the golden gate version of pSB1C3 ([https://parts.igem.org/Part:BBa_P10500 P10500]) using BsmBI or Esp3I and conforms to the [http://2016.igem.org/Resources/Plant_Synthetic_Biology/PhytoBricks Phytobrick standard].
+SP3_5_5 is cloned into the golden gate version of pSB1C3 ([https://parts.igem.org/Part:BBa_P10500 P10500]) using BsmBI or Esp3I and conforms to the [http://2016.igem.org/Resources/Plant_Synthetic_Biology/PhytoBricks Phytobrick standard].
-It is flanked by BsaI sites together 5' AATG and 3' GCTT cloning sequences for subsequent cloning into a level 1 plasmid.
+It is flanked by BsaI sites together 5' TACT and 3' CCAT cloning sequences for subsequent cloning into a level 1 plasmid.
-This sequence is incorporated into a level 1 plasmid for plant expression as part of an RNAi construct with the coding sequence found in [https://parts.igem.org/Part:BBa_K2810005  BBa_K2810005].
+This sequence is incorporated into a level 1 plasmid for plant expression as part of an RNAi construct with the complimentary sequence found in [https://parts.igem.org/Part:BBa_K2810008  BBa_K2810008].