Difference between revisions of "Part:BBa K2258003"

Latest revision as of 22:34, 1 November 2017

pCAT_AmilCP

It expresses AmilCP constitutively under pCAT promoter PCAT promoter (BBa_I14033) Composite Amil CP (BBa_K1357009)

Usage and Biology

This part is useful as constitutively expressed a reporter.

iGEM17_IISER-Pune-India: Expression of chromoproteins. The images above show E coli constitutively expressing RFP BBa_E1010 (red), tsPurple BBa_K1033906 (purple) and amilCP BBa_K592009 (blue).

Photometric Characterization

iGEM17_IISER-Pune-India: Absorbance of E.coli culture expressing AmilCP. The graph above shows photometric analysis of liquid culture of E.coli constitutively expressing AmilCP BBa_K592009 (blue) with an absorption peak at 590nm.

iGEM17_IISER-Pune-India: Absorbance of E.coli culture expressing AmilCP. The graph above shows photometric analysis of liquid culture of E.coli constitutively expressing AmilCP BBa_K592009 (blue) the absorbance at different optical densities are normalized with respect to the peak absorbance.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Revision as of 22:33, 1 November 2017 (view source) Jyothish (Talk \| contribs) ← Older edit		Latest revision as of 22:34, 1 November 2017 (view source) Jyothish (Talk \| contribs)
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−	[[Image:T--IISER-Pune-India--~~AmilCPabsorbance~~.jpg\|300px]]	+	[[Image:T--IISER-Pune-India--AmilCPabsorbancePeaknormallized.jpg\|300px]]

	'''iGEM17_IISER-Pune-India:''' Absorbance of ''E.coli'' culture expressing AmilCP. The graph above shows photometric analysis of liquid culture of ''E.coli'' constitutively expressing AmilCP <partinfo>BBa_K592009</partinfo> (blue) the absorbance at different optical densities are normalized with respect to the peak absorbance.		'''iGEM17_IISER-Pune-India:''' Absorbance of ''E.coli'' culture expressing AmilCP. The graph above shows photometric analysis of liquid culture of ''E.coli'' constitutively expressing AmilCP <partinfo>BBa_K592009</partinfo> (blue) the absorbance at different optical densities are normalized with respect to the peak absorbance.