Difference between revisions of "Part:BBa I732954"

Revision as of 01:25, 14 September 2008

Ptemplate1 + RBS.ARL4A0604

Genotype	Character	Name
Top10/pT-TERM	So not express any repressors	CR00
Top10/pT-ARL4A0604	Constitutively express ARL4A0604	CR01
Top10/pT-ARL2A0203	Constitutively express ARL2A0203	CR10
Top10/pTet-ARL4A0604-ARL2A203	Constitutively express ARL4A0604 and ARL2A0203	CR11

RS 01:25, 14 September 2008 (UTC): Note that this plasmid has been split into a plasmid backbone and part. The plasmid backbone is BBa_I732952 and the part is I732815. The physical DNA associated with this part has sequence BBa_I732952-I732815. This part needs to be deleted and the physical DNA remapped to the correct part number.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal suffix found in sequence at 1105
Illegal EcoRI site found at 3240
Illegal XbaI site found at 3405
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 3240
Illegal SpeI site found at 1106
Illegal PstI site found at 1120
Illegal NotI site found at 1113
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 3240
23
INCOMPATIBLE WITH RFC[23]
Illegal suffix found in sequence at 1106
Illegal EcoRI site found at 3240
Illegal XbaI site found at 3405
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 3240
Illegal XbaI site found at 3405
Illegal SpeI site found at 1106
Illegal PstI site found at 1120
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 2279
Illegal BsaI.rc site found at 3264

@@ Line 26: / Line 26: @@
 [[Image:USTC_plasmid_954.jpg]]
+*'''[[User:Rshetty|RS]] 01:25, 14 September 2008 (UTC)''': Note that this plasmid has been split into a plasmid backbone and part.  The plasmid backbone is BBa_I732952 and the part is I732815.  The physical DNA associated with this part has sequence BBa_I732952-I732815.  ''This part needs to be deleted and the physical DNA remapped to the correct part number.''
 <!-- Add more about the biology of this part here