Difference between revisions of "Part:BBa I732952"
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[[Image:USTC_plasmid_952.jpg]] | [[Image:USTC_plasmid_952.jpg]] | ||
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+ | *'''[[User:Rshetty|RS]] 01:03, 14 September 2008 (UTC)''': Note that this plasmid has been split into a plasmid backbone and part. The plasmid backbone is this part (BBa_I732952) and the part is BBa_I732807. The physical DNA associated with this part has sequence BBa_I732952-I732807 . | ||
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Revision as of 01:03, 14 September 2008
Ptemplate1 + RBS.ARL2A0103
Genotype | Character | Name |
Top10/pT-TERM | So not express any repressors | CR00 |
Top10/pT-ARL4A0604 | Constitutively express ARL4A0604 | CR01 |
Top10/pT-ARL2A0203 | Constitutively express ARL2A0203 | CR10 |
Top10/pTet-ARL4A0604-ARL2A203 | Constitutively express ARL4A0604 and ARL2A0203 | CR11 |
- RS 01:03, 14 September 2008 (UTC): Note that this plasmid has been split into a plasmid backbone and part. The plasmid backbone is this part (BBa_I732952) and the part is BBa_I732807. The physical DNA associated with this part has sequence BBa_I732952-I732807 .
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Plasmid lacks a prefix.
Illegal EcoRI site found at 2136
Illegal XbaI site found at 2301 - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2136
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2136 - 23INCOMPATIBLE WITH RFC[23]Plasmid lacks a prefix.
Illegal suffix found at 2
Illegal EcoRI site found at 2136
Illegal XbaI site found at 2301 - 25INCOMPATIBLE WITH RFC[25]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2136
Illegal XbaI site found at 2301
Illegal SpeI site found at 2
Illegal PstI site found at 16 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI.rc site found at 1175
Illegal BsaI.rc site found at 2160