Difference between revisions of "Part:BBa K2382013"
(→‎Improvemen)
Line 30: Line 30:
 
==Characterization of the EAAAK+ RFP+Histag==
 
==Characterization of the EAAAK+ RFP+Histag==
 
===Improvemen===
 
===Improvemen===
−
This is also an improvement of previous BioBrick Part <partinfo>BBa_E1010</partinfo>. It encodes an RFP that have
+
This is an improvement of previous BioBrick Part <partinfo>BBa_E1010</partinfo>. It encodes an RFP that have
 
the same amino acids as BBa_E1010 and having a His Tag at the end. Therefore, a fusion protein
 
the same amino acids as BBa_E1010 and having a His Tag at the end. Therefore, a fusion protein
 
with this part may have red color and the ability to be purified easily.
 
with this part may have red color and the ability to be purified easily.

Revision as of 02:05, 1 November 2017

EAAAK+ RFP+Histag


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 606
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology

This is a fragment of BBa_K2382010. We designed a restriction site, BamHI, before EAAAK rigid linker, so future iGEM teams could take advantage of this part to fuse their proteins with RFP as indicator.


Characterization of the EAAAK+ RFP+Histag

Improvemen

This is an improvement of previous BioBrick Part BBa_E1010. It encodes an RFP that have the same amino acids as BBa_E1010 and having a His Tag at the end. Therefore, a fusion protein with this part may have red color and the ability to be purified easily.

References

BioBrick Part BBa_E1010