Difference between revisions of "Part:BBa K2382013"
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This is also an improvement of previous BioBrick Part <partinfo>BBa_E1010</partinfo>. It encodes an RFP that have | This is also an improvement of previous BioBrick Part <partinfo>BBa_E1010</partinfo>. It encodes an RFP that have | ||
the same amino acids as BBa_E1010 and having a His Tag at the end. Therefore, a fusion protein | the same amino acids as BBa_E1010 and having a His Tag at the end. Therefore, a fusion protein | ||
Revision as of 02:01, 1 November 2017
EAAAK+ RFP+Histag
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 606
- 1000COMPATIBLE WITH RFC[1000]
Usage and Biology
This is a fragment of BBa_K2382010. We designed a restriction site, BamHI, before EAAAK rigid linker, so future iGEM teams could take advantage of this part to fuse their proteins with RFP as indicator.
Contents
Improvemen
This is also an improvement of previous BioBrick Part BBa_E1010. It encodes an RFP that have the same amino acids as BBa_E1010 and having a His Tag at the end. Therefore, a fusion protein with this part may have red color and the ability to be purified easily.
Characterization of the EAAAK+ RFP+Histag
References
BioBrick Part BBa_E1010