Difference between revisions of "Part:BBa K2374007"

Revision as of 17:27, 31 October 2017

UAS-TH -> (fruit fly)

TH
Use in	D.melanogaster
RFC standard	RFC 10 compatible
Backbone	pSB1C3
Submitted by	[http://2017.igem.org/Team:Tongji_China Tongji_China 2017]

ple (Tyrosine 3-monooxygenase, TH)

ple (TH) is a rate-limiting enzyme in the dopamine’s synthesis, and it plays an important role in the physiology of adrenergic neurons.
Catalytic activity: L-tyrosine + tetrahydrobiopterin + O2 = L-dopa + 4a-hydroxytetrahydrobiopterin.
This subpathway is part of the pathway dopamine biosynthesis, which is itself part of catecholamine biosynthesis.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 13
Illegal PstI site found at 256
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 13
Illegal PstI site found at 256
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1540
Illegal XhoI site found at 672
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 13
Illegal PstI site found at 256
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 13
Illegal PstI site found at 256
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 918
Illegal BsaI site found at 1884
Illegal BsaI.rc site found at 636
Illegal BsaI.rc site found at 1402
Illegal BsaI.rc site found at 1551

@@ Line 2: / Line 2: @@
 __NOTOC__
 <partinfo>BBa_K2374007 short</partinfo>
+{| style="color:black" cellpadding="6" cellspacing="1" border="2" align="right"
+! colspan="2" style="background:#FFBF00;"|TH
+|-
+|'''Use in'''
+|''D.melanogaster''
+|-
+|'''RFC standard'''
+|[https://parts.igem.org/Help:Assembly_standard_10 RFC 10] compatible
+|-
+|'''Backbone'''
+|pSB1C3<br>
+|-
+|'''Submitted by'''
+|[http://2017.igem.org/Team:Tongji_China Tongji_China 2017]
+|}
 '''ple''' (Tyrosine 3-monooxygenase, TH)