Difference between revisions of "Part:BBa K2374003"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K2374003 short</partinfo> | <partinfo>BBa_K2374003 short</partinfo> | ||
+ | |||
+ | {| style="color:black" cellpadding="6" cellspacing="1" border="2" align="right" | ||
+ | ! colspan="2" style="background:#FFBF00;"|pmyo2-ChR2-YFP | ||
+ | |- | ||
+ | |'''Use in''' | ||
+ | |''D.melanogaster'' | ||
+ | |- | ||
+ | |'''RFC standard''' | ||
+ | |[https://parts.igem.org/Help:Assembly_standard_10 RFC 10] compatible | ||
+ | |- | ||
+ | |'''Backbone''' | ||
+ | |pSB1C3<br> | ||
+ | |- | ||
+ | |'''Submitted by''' | ||
+ | |[http://2017.igem.org/Team:Tongji_China Tongji_China 2017] | ||
+ | |} | ||
+ | |||
Pale is a tyrosine hydroxylase, the first and rate-limiting step in the synthesis of dopamine (and eventually, melanin). Dopamine has critical roles in system development. We also provide Pale promoter in BBa_K2374001. | Pale is a tyrosine hydroxylase, the first and rate-limiting step in the synthesis of dopamine (and eventually, melanin). Dopamine has critical roles in system development. We also provide Pale promoter in BBa_K2374001. |
Revision as of 15:26, 29 October 2017
ple (Tyrosine 3-monooxygenase, TH) -> (fruit fly)
pmyo2-ChR2-YFP | |
---|---|
Use in | D.melanogaster |
RFC standard | RFC 10 compatible |
Backbone | pSB1C3 |
Submitted by | [http://2017.igem.org/Team:Tongji_China Tongji_China 2017] |
Pale is a tyrosine hydroxylase, the first and rate-limiting step in the synthesis of dopamine (and eventually, melanin). Dopamine has critical roles in system development. We also provide Pale promoter in BBa_K2374001.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1157
Illegal XhoI site found at 289 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 535
Illegal BsaI site found at 1501
Illegal BsaI.rc site found at 253
Illegal BsaI.rc site found at 1019
Illegal BsaI.rc site found at 1168
Functional Parameters
TH is involved in step 1 of the subpathway that synthesizes dopamine from L-tyrosine and plays an important role in the physiology of adrenergic neurons.