Difference between revisions of "Part:BBa K2446039"
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<partinfo>BBa_K2446039 short</partinfo> | <partinfo>BBa_K2446039 short</partinfo> | ||
− | + | <div> | |
+ | This part is one of our mammalian synthetic transcription factors (SynTFs) based on Cys2-His2 zinc finger 21-16 (ZF_21-16) as protein chassis. [[#References|[1]]]. ZF_21-16_KRAB (or TF-KRAB-3 in our project) containing three core domains from N-terminal to C-terminal: ZF_21-16 DNA binding domain, nuclear location sequence and KRAB transcription regulating domain [[#References|[2]]].And a (G4S) linker was added between DBD and NLS for providing region flexibility [[#References|[3]]]. ZF_21-16-DBD enable binding to specific DNA sequences(as figure 1 show), so that we can use ZF_21-16-KRAB as a specific transcription factors to repress the expression of our mammalian synthetic promoter SV40-8_ZF_21-16 ( <partinfo>BBa_K2446030</partinfo>). | ||
+ | </div> | ||
+ | [[file:T--Fudan--design%26ZF.png|800px|middle|'''Figure 1''':(A)Design of SynTF-SynPros. (B) The specific binding sites sequences of Zinc finger we used]] | ||
+ | <div> | ||
+ | <B> Figure 1:(A)Design of SynTF-SynPros. (B) The specific binding sites sequences of Zinc finger we used</B> | ||
+ | </div> | ||
+ | The information of other SynTF-SynPros is showed in the table below. | ||
+ | <table border="2" width="800px" > | ||
+ | <tr> | ||
+ | <th>SynTFs </th> | ||
+ | <th>SynPros</th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>Gal4-KRAB(TF-KRAB-1) <partinfo>BBa_K2446037</partinfo></td> | ||
+ | <td>Sv40-UAS(Sv40-UAS) <partinfo>BBa_K2446036</partinfo></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>ZF_PIP_KRAB(TF-KRAB-2) <partinfo>BBa_K2446045</partinfo></td> | ||
+ | <td>SV40_2/4/8_PIP <partinfo>BBa_K2446033</partinfo>/<partinfo>BBa_K2446034</partinfo>/<partinfo>BBa_K2446035</partinfo></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>ZF_21-16KRAB(TF-KRAB-3) <partinfo>BBa_K2446039 </partinfo></td> | ||
+ | <td>SV40_8_ZF_21-16 <partinfo>BBa_K2446030</partinfo></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>ZF_42-10_KRAB(TF-KRAB-4) <partinfo>BBa_K2446040</partinfo></td> | ||
+ | <td>SV40_8_ZF_42-10 <partinfo>BBa_K2446025</partinfo></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>ZF_43-8_KRAB(TF-KRAB-5) <partinfo>BBa_K2446041</partinfo></td> | ||
+ | <td>SV40_2/4/8_ZF_43-8 <partinfo>BBa_K2446026</partinfo>/<partinfo>BBa_K2446027</partinfo>/<partinfo>BBa_K2446028</partinfo></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>ZF_54-8_KRAB(TF-KRAB-6) <partinfo>BBa_K2446042</partinfo></td> | ||
+ | <td>SV40_8_ZF_54-8 <partinfo>BBa_K2446029</partinfo></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>ZFHD1_KRAB(TF-KRAB-7) <partinfo>BBa_K2446043</partinfo></td> | ||
+ | <td>SV40_4_ZFHD1 <partinfo>BBa_K2446032</partinfo></td> | ||
+ | </tr> | ||
+ | </table> | ||
+ | |||
+ | |||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 04:09, 31 October 2017
ZF_21-16KRAB
This part is one of our mammalian synthetic transcription factors (SynTFs) based on Cys2-His2 zinc finger 21-16 (ZF_21-16) as protein chassis. [1]. ZF_21-16_KRAB (or TF-KRAB-3 in our project) containing three core domains from N-terminal to C-terminal: ZF_21-16 DNA binding domain, nuclear location sequence and KRAB transcription regulating domain [2].And a (G4S) linker was added between DBD and NLS for providing region flexibility [3]. ZF_21-16-DBD enable binding to specific DNA sequences(as figure 1 show), so that we can use ZF_21-16-KRAB as a specific transcription factors to repress the expression of our mammalian synthetic promoter SV40-8_ZF_21-16 ( BBa_K2446030).
Figure 1:(A)Design of SynTF-SynPros. (B) The specific binding sites sequences of Zinc finger we used
The information of other SynTF-SynPros is showed in the table below.
SynTFs | SynPros |
---|---|
Gal4-KRAB(TF-KRAB-1) BBa_K2446037 | Sv40-UAS(Sv40-UAS) BBa_K2446036 |
ZF_PIP_KRAB(TF-KRAB-2) BBa_K2446045 | SV40_2/4/8_PIP BBa_K2446033/BBa_K2446034/BBa_K2446035 |
ZF_21-16KRAB(TF-KRAB-3) BBa_K2446039 | SV40_8_ZF_21-16 BBa_K2446030 |
ZF_42-10_KRAB(TF-KRAB-4) BBa_K2446040 | SV40_8_ZF_42-10 BBa_K2446025 |
ZF_43-8_KRAB(TF-KRAB-5) BBa_K2446041 | SV40_2/4/8_ZF_43-8 BBa_K2446026/BBa_K2446027/BBa_K2446028 |
ZF_54-8_KRAB(TF-KRAB-6) BBa_K2446042 | SV40_8_ZF_54-8 BBa_K2446029 |
ZFHD1_KRAB(TF-KRAB-7) BBa_K2446043 | SV40_4_ZFHD1 BBa_K2446032 |
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 301
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]