Difference between revisions of "Part:BBa I739021"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_I739021 short</partinfo>
 
<partinfo>BBa_I739021 short</partinfo>
  
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===Part Structure===
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<p>The Biobrick encodes [https://parts.igem.org/wiki/index.php/Help:Tag LVA]-tagged P22 cII ([https://parts.igem.org/wiki/index.php/Part:BBa_C0053 BBa_C0053]) and [https://parts.igem.org/wiki/index.php/Help:Tag LVA]-tagged EYFP ([https://parts.igem.org/wiki/index.php/Part:BBa_E0032 BBa_E0032]) under control of the double promoter [https://parts.igem.org/wiki/index.php/Part:BBa_I739101 BBa_I739101] followed by the ribosome binding site [https://parts.igem.org/wiki/index.php/Part:BBa_B0034 BBa_B0034]. The transcription of P22 cII and EYFP is terminated by the double terminator [https://parts.igem.org/wiki/index.php/Part:BBa_B0015 BBa_B0015].</p>
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===Mode of Action===
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<p>P22 cII and EYFP production can be repressed by tetR. If the inducer [http://openwetware.org/wiki/ATc anhydrotetracycline (ATc)] is added, tetR action is inhibited and the promoter gets derepressed.</p>
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===Purpose===
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<p>This Biobrick was designed for the [http://parts.mit.edu/igem07/index.php/ETHZ ETHZ iGEM 2007 project] and is a composite biobrick including a first generation double promoter. The goal is to show the applicability of such kind of double promoters. ...
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===Testing===
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<p>Checked for uniqueness of restriction enzyme cleavage sites:<br>
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Eco: ok<br>
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Xba: ok<br>
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Spe: ok<br>
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Pst: ok<br>
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</p>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 12:10, 19 October 2007

Single regulated polycistronic P22 cII +LVA / EYFP +LVA expression cassette

Part Structure

The Biobrick encodes LVA-tagged P22 cII (BBa_C0053) and LVA-tagged EYFP (BBa_E0032) under control of the double promoter BBa_I739101 followed by the ribosome binding site BBa_B0034. The transcription of P22 cII and EYFP is terminated by the double terminator BBa_B0015.

Mode of Action

P22 cII and EYFP production can be repressed by tetR. If the inducer [http://openwetware.org/wiki/ATc anhydrotetracycline (ATc)] is added, tetR action is inhibited and the promoter gets derepressed.

Purpose

This Biobrick was designed for the [http://parts.mit.edu/igem07/index.php/ETHZ ETHZ iGEM 2007 project] and is a composite biobrick including a first generation double promoter. The goal is to show the applicability of such kind of double promoters. ...

Testing

<p>Checked for uniqueness of restriction enzyme cleavage sites:
Eco: ok
Xba: ok
Spe: ok
Pst: ok

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 275