Difference between revisions of "Part:BBa I739005"
Stefan Luzi (Talk | contribs) (→Purpose) |
Stefan Luzi (Talk | contribs) (→Part Structure) |
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===Part Structure=== | ===Part Structure=== | ||
<p>The Biobrick encodes [https://parts.igem.org/wiki/index.php/Help:Tag LVA]-tagged EYFP ([https://parts.igem.org/wiki/index.php/Part:BBa_E0032 BBa_E0032]) downstream of the ribosome binding site [https://parts.igem.org/wiki/index.php/Part:BBa_B0034 BBa_B0034]. The transcription of eYFP is terminated by the double terminator [https://parts.igem.org/wiki/index.php/Part:BBa_B0015 BBa_B0015]. This construct can be used as second cistron expressing a reporter gene in a multicistronic construct. | <p>The Biobrick encodes [https://parts.igem.org/wiki/index.php/Help:Tag LVA]-tagged EYFP ([https://parts.igem.org/wiki/index.php/Part:BBa_E0032 BBa_E0032]) downstream of the ribosome binding site [https://parts.igem.org/wiki/index.php/Part:BBa_B0034 BBa_B0034]. The transcription of eYFP is terminated by the double terminator [https://parts.igem.org/wiki/index.php/Part:BBa_B0015 BBa_B0015]. This construct can be used as second cistron expressing a reporter gene in a multicistronic construct. | ||
| − | <br><br> | + | <br><br>''WARNING:'' |
This part is identical to part [https://parts.igem.org/wiki/index.php/Part:BBa_E0432 BBa_E0432]. However, since BBa_E0432 is not properly working, it was reconstructed and retested. If there should still be issues with this part, one should switch to [https://parts.igem.org/wiki/index.php/Part:BBa_E0430 BBa_E0430] or [https://parts.igem.org/wiki/index.php/Part:BBa_E0434 BBa_E0434]. Because part [https://parts.igem.org/wiki/index.php/Part:BBa_E0438 BBa_E0438] seems to be difficult to handle too, on could conclude that the LVA-tagged EYFP (BBa_E0032) is not working at all! So please be careful with those parts...</p> | This part is identical to part [https://parts.igem.org/wiki/index.php/Part:BBa_E0432 BBa_E0432]. However, since BBa_E0432 is not properly working, it was reconstructed and retested. If there should still be issues with this part, one should switch to [https://parts.igem.org/wiki/index.php/Part:BBa_E0430 BBa_E0430] or [https://parts.igem.org/wiki/index.php/Part:BBa_E0434 BBa_E0434]. Because part [https://parts.igem.org/wiki/index.php/Part:BBa_E0438 BBa_E0438] seems to be difficult to handle too, on could conclude that the LVA-tagged EYFP (BBa_E0032) is not working at all! So please be careful with those parts...</p> | ||
Revision as of 09:26, 12 October 2007
Promoterless expression cassette for EYFP +LVA (B0034.E0032.B0015)
Part Structure
The Biobrick encodes LVA-tagged EYFP (BBa_E0032) downstream of the ribosome binding site BBa_B0034. The transcription of eYFP is terminated by the double terminator BBa_B0015. This construct can be used as second cistron expressing a reporter gene in a multicistronic construct.
WARNING:
This part is identical to part BBa_E0432. However, since BBa_E0432 is not properly working, it was reconstructed and retested. If there should still be issues with this part, one should switch to BBa_E0430 or BBa_E0434. Because part BBa_E0438 seems to be difficult to handle too, on could conclude that the LVA-tagged EYFP (BBa_E0032) is not working at all! So please be careful with those parts...
Mode of Action
Enhanced yellow fluorescent proteins (EYFP) are fluorochromes, commonly used as reporter proteins.
Purpose
This Biobrick was designed for the [http://parts.mit.edu/igem07/index.php/ETHZ ETHZ iGEM 2007 project] and belongs to the constitutive part of the system. In the project description, this part is also termed Part 5. ....
Testing
Checked for uniqueness of restriction enzyme cleavage sites:
Eco: ok
Xba: ok
Spe: ok
Pst: ok
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]