Difference between revisions of "Part:BBa I739005"

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===Mode of Action===
 
===Mode of Action===
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<p>TetR binds to the pTet regulator ([https://parts.igem.org/wiki/index.php/Part:BBa_R0040 BBa_R0040)] and represses transcription. If the inducer [http://openwetware.org/wiki/ATc anhydrotetracycline (ATc)] is added, tetR action is inhibited and the promoter gets derepressed.</p>
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<p>Enhanced yellow fluorescent protein (eYFP) are fluorochromes with an excitation wavelength of </p>
  
 
===Purpose===
 
===Purpose===

Revision as of 07:59, 12 October 2007

Promoterless expression cassette for EYFP +LVA (B0034.E0032.B0015)

Part Structure

The Biobrick encodes LVA-tagged eYFP (BBa_E0032) downstream of the ribosome binding site BBa_B0034. The transcription of eYFP is terminated by the double terminator BBa_B0015. This construct can be used as second cistron expressing a reporter gene in a multicistronic construct.

Mode of Action

Enhanced yellow fluorescent protein (eYFP) are fluorochromes with an excitation wavelength of

Purpose

This Biobrick was designed for the [http://parts.mit.edu/igem07/index.php/ETHZ ETHZ iGEM 2007 project] and belongs to the constitutive part of the system. In the project description, this part is also termed Part 1. The constitutively synthesized TetR interacts with .... A similar (but not used) construct in this context is BBa_Q04400.

Testing

Checked for uniqueness of restriction enzyme cleavage sites:
Eco: ok
Xba: ok
Spe: ok
Pst: ok


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]