Difference between revisions of "Part:BBa K2120309"
 
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<br>(1) Arabinose induced promoter pBAD([https://parts.igem.org/Part:BBa_K2120305 BBa_K2120305]) which we reserve the parts [https://parts.igem.org/Part:BBa_K808000 BBa_K808000].This part contains the promoter as well as the coding sequence for the repressor gene ''araC'' which is transcribed in the opposite direction(“upstream”). By binding to L(+)-arabinose, AraC changes its conformation. This causes the protein to diffuses from the DNA thereby inducing transcription.
 
<br>(1) Arabinose induced promoter pBAD([https://parts.igem.org/Part:BBa_K2120305 BBa_K2120305]) which we reserve the parts [https://parts.igem.org/Part:BBa_K808000 BBa_K808000].This part contains the promoter as well as the coding sequence for the repressor gene ''araC'' which is transcribed in the opposite direction(“upstream”). By binding to L(+)-arabinose, AraC changes its conformation. This causes the protein to diffuses from the DNA thereby inducing transcription.
 
<br>(2)The pR promoter's inhibitor gene ''cI'' from ''bacteriophage lambda''.
 
<br>(2)The pR promoter's inhibitor gene ''cI'' from ''bacteriophage lambda''.
−
<br>(3)''RFP'', as the report gene, can verify the pLac promoter's activity. We opposite the two promoters’ direction for reducing the interfluence of two promoters.
+
<br>(3)''RFP'', as the report gene, can verify the pLac promoter's activity. We opposite the two promoters’ direction for reducing the influence of two promoters.
  
 
    
 
    

Latest revision as of 17:34, 22 October 2016


B0015+CI+B0034+pBAD-AraC+pR+B0032+RFP+B0015

<Description>
This part is made of three elements:
(1) Arabinose induced promoter pBAD(BBa_K2120305) which we reserve the parts BBa_K808000.This part contains the promoter as well as the coding sequence for the repressor gene araC which is transcribed in the opposite direction(“upstream”). By binding to L(+)-arabinose, AraC changes its conformation. This causes the protein to diffuses from the DNA thereby inducing transcription.
(2)The pR promoter's inhibitor gene cI from bacteriophage lambda.
(3)RFP, as the report gene, can verify the pLac promoter's activity. We opposite the two promoters’ direction for reducing the influence of two promoters.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1001
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1166
    Illegal AgeI site found at 2788
    Illegal AgeI site found at 2900
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1183