Difference between revisions of "Part:BBa K2120303"

Revision as of 17:03, 22 October 2016

araC-pBAD+B0034+tetR+B0015

<Description></br> This part is composed of two elements:</br> (1) Arabinose induced promoter pBAD and its inhibitor gene araC BBa_K808000.This part contains the promoter as well as the coding sequence for the repressor araC which is transcribed in the opposite direction. (“upstream”) By binding to L(+)-arabinose, araC changes its conformation. This causes the protein to diffuse from the DNA thereby inducing transcription.</br> (2) Tetracyclines induced promoter pTet's inhibitor gene tetR.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1144
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 979
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 961

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2120303 short</partinfo>
-'''<Description>'''
+'''<Description>'''</br>
-This part is composed of two elements:
+This part is composed of two elements:</br>
-(1) Arabinose induced promoter pBAD and its inhibitor gene ''araC'' [https://parts.igem.org/Part:BBa_K808000 BBa_K808000].This part contains the promoter as well as the coding sequence for the repressor araC which is transcribed in the opposite direction. (“upstream”) By binding to L(+)-arabinose, araC changes its conformation. This causes the protein to diffuse from the DNA thereby inducing transcription.
+(1) Arabinose induced promoter pBAD and its inhibitor gene ''araC'' [https://parts.igem.org/Part:BBa_K808000 BBa_K808000].This part contains the promoter as well as the coding sequence for the repressor araC which is transcribed in the opposite direction. (“upstream”) By binding to L(+)-arabinose, araC changes its conformation. This causes the protein to diffuse from the DNA thereby inducing transcription.</br>
 (2) Tetracyclines induced promoter pTet's inhibitor gene ''tetR''.