Difference between revisions of "Part:BBa K1897020"
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− | The construct contains lldRO1-J23117-lldRO2 promoter designed by team ETH-Zurich 2015 ([[Part:BBa_K1847008]]) with a medium RBS flanked by non-coding sequences ([[Part:BBa_K1897032]]), followed by a | + | The construct contains lldRO1-J23117-lldRO2 promoter designed by team ETH-Zurich 2015 ([[Part:BBa_K1847008]]) with a medium RBS flanked by non-coding sequences ([[Part:BBa_K1897032]]), followed by a coding sequence of ALR ([[Part:BBa_K1172901]]) and a lambda t0 terminator ([[Part:BBa_K1897030]]). |
Revision as of 18:27, 17 October 2016
p70-32-ALR-Terminator
The construct contains lldRO1-J23117-lldRO2 promoter designed by team ETH-Zurich 2015 (Part:BBa_K1847008) with a medium RBS flanked by non-coding sequences (Part:BBa_K1897032), followed by a coding sequence of ALR (Part:BBa_K1172901) and a lambda t0 terminator (Part:BBa_K1897030).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 78
Illegal NheI site found at 101
Illegal NheI site found at 501 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 425
Illegal BamHI site found at 1127 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 543
Illegal AgeI site found at 843 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 300