Difference between revisions of "Part:BBa K1951009"
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Flagellin C (FliC) protein from <i>Desulfovibrio vulgaris</i> strain is the main protein constitutive of the flagellum filament and is involved to promote bacterial swimming<ref>Capeness & al. 2015, http://eprints.nottingham.ac.uk/27979/1/Michael%20Capeness%20-%20Thesis%20-%20PDF.pdf</ref>. This sequence is conserved in many bacterial strains as the capacity of swimming given by the flagellum confers a great selective advantage. | Flagellin C (FliC) protein from <i>Desulfovibrio vulgaris</i> strain is the main protein constitutive of the flagellum filament and is involved to promote bacterial swimming<ref>Capeness & al. 2015, http://eprints.nottingham.ac.uk/27979/1/Michael%20Capeness%20-%20Thesis%20-%20PDF.pdf</ref>. This sequence is conserved in many bacterial strains as the capacity of swimming given by the flagellum confers a great selective advantage. | ||
| − | ==Metal Biosorption Capacity== | + | ===Metal Biosorption Capacity=== |
It has been demonstrated that Flagellin has the ability to adsorb precious metal on its surface such as platinum, palladium gold <ref>Deplanche & al., 2007 http://onlinelibrary.wiley.com/doi/10.1002/bit.21688/abstract. </ref>... | It has been demonstrated that Flagellin has the ability to adsorb precious metal on its surface such as platinum, palladium gold <ref>Deplanche & al., 2007 http://onlinelibrary.wiley.com/doi/10.1002/bit.21688/abstract. </ref>... | ||
Revision as of 21:41, 15 October 2016
FliC Desulfovibrio producer
FliC, the main swimming protein (K1951006 + K880005)
General
Flagellin C (FliC) protein from Desulfovibrio vulgaris strain is the main protein constitutive of the flagellum filament and is involved to promote bacterial swimming[1]. This sequence is conserved in many bacterial strains as the capacity of swimming given by the flagellum confers a great selective advantage.
Metal Biosorption Capacity
It has been demonstrated that Flagellin has the ability to adsorb precious metal on its surface such as platinum, palladium gold [2]...
We assess the production of the protein by a SDS page/comassie test. We made a FliC mutant by transduction using phage P1 in a E. Coli W3110 strain.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 362
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 461