Difference between revisions of "Part:BBa K1992000"

Revision as of 16:50, 15 October 2016

Tar native RBS

Usage and biology

This RBS obtained from the genome of E.coli K12 and located upstream to Tar encoding DNA sequence. The main use of this RBS is to examine its effect on the expression level of Tar chemoreceptor compared to the strongest RBS (B0034) according to the [http://2010.igem.org/Team:Warsaw/Stage1/RBSMeas Warsaw 2010's measurement].

Experiments and results

In order to compare two the RBSs two expression plasmids constructed containing BBa_J23100 as promoter, the tested RBS, Tar receptor BBa_K777000 and terminator BBa_B0015. The two plasmids BBa_K1992004 and BBa_K1992005 were cloned to chemoreceptor free E.coli strain (UU1250) and tested by swarming assay as can be seen in figure 1.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 ===Usage and biology===
 This RBS obtained from the genome of E.coli K12 and located upstream to Tar encoding DNA sequence. The main use of this RBS is to examine its effect on the expression level of Tar chemoreceptor compared to the strongest RBS (B0034) according to the [http://2010.igem.org/Team:Warsaw/Stage1/RBSMeas Warsaw 2010's measurement].
+===Experiments and results===
+In order to compare two the RBSs two expression plasmids constructed containing <partinfo>BBa_J23100</partinfo> as promoter, the tested RBS, Tar receptor <partinfo>BBa_K777000</partinfo> and terminator <partinfo>BBa_B0015</partinfo>. The two plasmids <partinfo>BBa_K1992004</partinfo> and <partinfo>BBa_K1992005</partinfo> were cloned to chemoreceptor free E.coli strain (UU1250) and tested by swarming assay as can be seen in figure 1.
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