Difference between revisions of "Part:BBa K1890020"

Revision as of 13:19, 9 October 2016

GFP with strong constitutive promoter, RBS and terminator

Indroduction

Green fluorescent protein (GFP) from the jellyfish Aequorea victoria. This mutant represents a series of mutations resulting in enhanced maturation and emission [1]. It is expressed under control of the strong constitutive promoter BBa_J23100, strong RBS BBa_B0030 and terminators BBa_B0010 and BBa_B0012.

Construction

This part is based on the part BBa_E0840, which already contains RBS, GFP gene and terminators. By means of PCR we added the strong constitutive promoter BBa_J23100.

<figure> <img src="" width="70%" height="100%"> <figcaption> Figure 1: Construction of the biobrick K1890020 by means of two PCRs, using biobrick E0840 as a template. </figcaption> </figure>

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 700

References

[1] Cormack, B. P., Valdivia, R. H., & Falkow, S. (1996). FACS-optimized mutants of the green fluorescent protein (GFP). Gene, 173(1), 33-38.

@@ Line 8: / Line 8: @@
 <h2>Construction</h2>
 This part is based on the part <partinfo>BBa_E0840</partinfo>, which already contains RBS, GFP gene and terminators. By means of PCR we added the strong constitutive promoter <partinfo>BBa_J23100</partinfo>.
+<figure>
+<img src="https://static.igem.org/mediawiki/2016/4/45/T--TU_Delft--BBa_K1890020_construction.png" width="70%" height="100%">
+<figcaption>
+<b>Figure 1</b>: Construction of the biobrick K1890020 by means of two PCRs, using biobrick E0840 as a template.
+</figcaption>
+</figure>
 <h2><span class='h3bb'>Sequence and Features</span></h2>