Difference between revisions of "Part:BBa K1890020"

 
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<partinfo>BBa_K1890020 short</partinfo>
 
<partinfo>BBa_K1890020 short</partinfo>
  
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Green fluorescent protein (GFP) from the jellyfish <i>Aequorea victoria</i>. This mutant represents a series of mutations resulting in enhanced maturation and emission. It is expressed under the strong constitutive promoter J23100.  
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<h2>Indroduction</h2>
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Green fluorescent protein (GFP) from the jellyfish <i>Aequorea victoria</i>. This mutant represents a series of mutations resulting in enhanced maturation and emission. It is expressed under control of the strong constitutive promoter <partinfo>BBa_J23100</partinfo>, strong RBS <partinfo>BBa_B0030</partinfo> and terminators <partinfo>BBa_B0010</partinfo> and <partinfo>BBa_B0012</partinfo>.
  
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<h2>Construction</h2>
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===Usage and Biology===
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This part is based on the part <partinfo>BBa_B0840</partinfo>, already containing RBS, GFP gene and terminators. By means of PCR we added the strong constitutive promoter <partinfo>BBa_J23100</partinfo>.
  
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<h2><span class='h3bb'>Sequence and Features</span></h2>
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K1890020 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1890020 SequenceAndFeatures</partinfo>
  
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<h2>References</h2>
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[1] Cormack, B. P., Valdivia, R. H., & Falkow, S. (1996). FACS-optimized mutants of the green fluorescent protein (GFP). Gene, 173(1), 33-38.
  
 
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Revision as of 12:59, 9 October 2016


GFP with strong constitutive promoter, RBS and terminator

Indroduction

Green fluorescent protein (GFP) from the jellyfish Aequorea victoria. This mutant represents a series of mutations resulting in enhanced maturation and emission. It is expressed under control of the strong constitutive promoter BBa_J23100, strong RBS BBa_B0030 and terminators BBa_B0010 and BBa_B0012.

Construction

This part is based on the part No part name specified with partinfo tag., already containing RBS, GFP gene and terminators. By means of PCR we added the strong constitutive promoter BBa_J23100.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 700

References

[1] Cormack, B. P., Valdivia, R. H., & Falkow, S. (1996). FACS-optimized mutants of the green fluorescent protein (GFP). Gene, 173(1), 33-38.