Difference between revisions of "Part:BBa K1982009"
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Cryptochrome 2 (CRY2) is a blue light stimulated photoreceptor, when exposed to blue light, it would interact withthe N-terminal fragment of CIB1 (CIBN) . This part is full-length CRY2 CDS.
 
Cryptochrome 2 (CRY2) is a blue light stimulated photoreceptor, when exposed to blue light, it would interact withthe N-terminal fragment of CIB1 (CIBN) . This part is full-length CRY2 CDS.
 
Optogenetic systems enable precise spatial and temporal control of cell behavior. A light-activated CRISPR/Cas9 effector (LACE) system that induces transcription of endogenous genes in the presence of blue light.This was accomplished by fusing the light-inducible heterodimerizing proteins CRY2 and CIB1 to a transactivation domain and the catalytically inactive tCas9, respectively. The versatile LACE system can be easily directed to new DNA sequences for the dynamic regulation of endogenous genes[1].
 
Optogenetic systems enable precise spatial and temporal control of cell behavior. A light-activated CRISPR/Cas9 effector (LACE) system that induces transcription of endogenous genes in the presence of blue light.This was accomplished by fusing the light-inducible heterodimerizing proteins CRY2 and CIB1 to a transactivation domain and the catalytically inactive tCas9, respectively. The versatile LACE system can be easily directed to new DNA sequences for the dynamic regulation of endogenous genes[1].

Revision as of 06:17, 9 October 2016


Eukaryotic Cryptochrome 2 (CRY2) ( a blue light stimulated photoreceptor)

CRY2
Function blue light stimulated photoreceptor
RFC standard RFC 10
Backbone pSB1C3
Organism Arabidopsis thaliana
Source Arabidopsis thaliana
Submitted by [http://2016.igem.org/Team:NEU-China NEU-China 2016]


Cryptochrome 2 (CRY2) is a blue light stimulated photoreceptor, when exposed to blue light, it would interact withthe N-terminal fragment of CIB1 (CIBN) . This part is full-length CRY2 CDS. Optogenetic systems enable precise spatial and temporal control of cell behavior. A light-activated CRISPR/Cas9 effector (LACE) system that induces transcription of endogenous genes in the presence of blue light.This was accomplished by fusing the light-inducible heterodimerizing proteins CRY2 and CIB1 to a transactivation domain and the catalytically inactive tCas9, respectively. The versatile LACE system can be easily directed to new DNA sequences for the dynamic regulation of endogenous genes[1].


Protein data table for BioBrick BBa_ automatically created by the BioBrick-AutoAnnotator version 1.0
Nucleotide sequence in RFC 10: (underlined part encodes the protein)
 ATGAAGATG ... GGTTGCAAATAATAA
 ORF from nucleotide position 1 to 1836 (excluding stop-codon)
Amino acid sequence: (RFC 25 scars in shown in bold, other sequence features underlined; both given below)
1 
101 
201 
301 
401 
501 
601 		MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQFYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVF
NHLYDPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSIESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEK
PSNALLTRAWSPGWSNADKLLNEFIEKQLIDYAKNSKKVVGNCTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGEESADLFLRGIGLREYSRYI
CFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPLVDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQYI
SGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKASGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAAPD
EIVADSFEALGANTIKEPGLCPSVSSNDQQVPSAVRYNGSKRVKPEEEEERDMKKSRGFDERELFSTAESSSSSSVFFVSQSCSLASEGKNLEGIQDSSD
QITTSLGKNGCK*
Sequence features: (with their position in the amino acid sequence, see the list of supported features)
None of the supported features appeared in the sequence
Amino acid composition:
Ala (A)47 (7.7%)
Arg (R)33 (5.4%)
Asn (N)21 (3.4%)
Asp (D)35 (5.7%)
Cys (C)12 (2.0%)
Gln (Q)18 (2.9%)
Glu (E)48 (7.8%)
Gly (G)36 (5.9%)
His (H)13 (2.1%)
Ile (I)36 (5.9%)
Leu (L)59 (9.6%)
Lys (K)38 (6.2%)
Met (M)12 (2.0%)
Phe (F)25 (4.1%)
Pro (P)31 (5.1%)
Ser (S)54 (8.8%)
Thr (T)24 (3.9%)
Trp (W)22 (3.6%)
Tyr (Y)17 (2.8%)
Val (V)31 (5.1%)
Amino acid counting
Total number:612
Positively charged (Arg+Lys):71 (11.6%)
Negatively charged (Asp+Glu):83 (13.6%)
Aromatic (Phe+His+Try+Tyr):77 (12.6%)
Biochemical parameters
Atomic composition:C3125H4814N836O913S24
Molecular mass [Da]:69473.1
Theoretical pI:5.69
Extinction coefficient at 280 nm [M-1 cm-1]:146330 / 147080 (all Cys red/ox)
Plot for hydrophobicity, charge, predicted secondary structure, solvent accessability, transmembrane helices and disulfid bridges 
Codon usage
Organism:E. coliB. subtilisS. cerevisiaeA. thalianaP. patensMammals
Codon quality (CAI):good (0.69)good (0.73)good (0.69)good (0.78)good (0.80)good (0.67)
Alignments (obtained from PredictProtein.org)
   There were no alignments for this protein in the data base. The BLAST search was initialized and should be ready in a few hours.
Predictions (obtained from PredictProtein.org)
   There were no predictions for this protein in the data base. The prediction was initialized and should be ready in a few hours.
The BioBrick-AutoAnnotator was created by TU-Munich 2013 iGEM team. For more information please see the documentation.
If you have any questions, comments or suggestions, please leave us a comment.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 405
    Illegal BglII site found at 864
    Illegal BamHI site found at 1343
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 289
    Illegal AgeI site found at 1018
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 641
    Illegal BsaI.rc site found at 50
    Illegal SapI.rc site found at 158