Difference between revisions of "Part:BBa K1890010"
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<h2>Indroduction</h2> | <h2>Indroduction</h2> | ||
| − | mCerulean is a cyan fluorescent protein derived from ECFP. It presents a series of mutations that increase its extinction coefficient, quantum yield and fluorescence lifetime. We expressed it under the control of the strong constitutive promoter <partinfo>BBa_J23100</partinfo> and strong RBS <partinfo>BBa_B0030</partinfo>. | + | mCerulean is a cyan fluorescent protein derived from ECFP. It presents a series of mutations that increase its extinction coefficient, quantum yield and fluorescence lifetime [1]. We expressed it under the control of the strong constitutive promoter <partinfo>BBa_J23100</partinfo> and strong RBS <partinfo>BBa_B0030</partinfo>. |
<h2>Sequence and Features</h2> | <h2>Sequence and Features</h2> | ||
<partinfo>BBa_K1890010 SequenceAndFeatures</partinfo> | <partinfo>BBa_K1890010 SequenceAndFeatures</partinfo> | ||
| + | |||
| + | <h2>References</h2> | ||
| + | [1] Rizzo, M. a, Springer, G. H., Granada, B. & Piston, D. W. An improved cyan fluorescent protein variant useful for FRET. Nat. Biotechnol. 22, 445–449 (2004). | ||
Revision as of 15:21, 7 October 2016
mCerulean with strong consitutive promoter and RBS
Indroduction
mCerulean is a cyan fluorescent protein derived from ECFP. It presents a series of mutations that increase its extinction coefficient, quantum yield and fluorescence lifetime [1]. We expressed it under the control of the strong constitutive promoter BBa_J23100 and strong RBS BBa_B0030.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
[1] Rizzo, M. a, Springer, G. H., Granada, B. & Piston, D. W. An improved cyan fluorescent protein variant useful for FRET. Nat. Biotechnol. 22, 445–449 (2004).