Difference between revisions of "Part:pSB4K5:Experience"
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<I>Reshma Shetty</I>
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<I>[[User:Rshetty|Reshma Shetty]]</I>
 
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When miniprepped, pSB4K5-I52001 gives high yield similar to a high copy plasmid.  I also successfully used this plasmid to clone 3 constructs that I could not assemble using either pSB1AC3 or pSB3K5.  (I think the lower copy number of the plasmid was key to this construction working.)
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When miniprepped, [[Part:pSB4K5|pSB4K5]]-[[Part:BBa_I52002|I52002]] gives high miniprep yield similar to a high copy plasmid.  I also successfully used this plasmid to clone 3 constructs that I could not assemble using either [[Part:pSB1AC3|pSB1AC3]] or [[Part:pSB3K5|pSB3K5]].  (I think the lower copy number of the plasmid was key to this construction working.)
 
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Revision as of 00:49, 25 January 2008

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of pSB4K5

User Reviews

UNIQ2c16294a2ff61729-partinfo-00000000-QINU

•••••

Reshma Shetty

When miniprepped, pSB4K5-I52002 gives high miniprep yield similar to a high copy plasmid. I also successfully used this plasmid to clone 3 constructs that I could not assemble using either pSB1AC3 or pSB3K5. (I think the lower copy number of the plasmid was key to this construction working.)

UNIQ2c16294a2ff61729-partinfo-00000002-QINU