Difference between revisions of "Part:BBa K1602055"
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<partinfo>BBa_K1602055 short</partinfo>
 
<partinfo>BBa_K1602055 short</partinfo>
  
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Composite part consisting of an araC-regulated pBAD-promoter (<html><a href="/Part:BBa_K808000">BBa_K808000</a></html>) cloned upstream of a ribosome binding site (<html><a href="/Part:BBa_B0034">BBa_B0034</a></html>) and GFP (<html><a href="/Part:BBa_E0040">BBa_E0040</a></html>). Because of the <html><a href="/Part:BBa_K808000">araC-regulated pBAD-promoter</a></html> production of the GFP is induced by the addition of arabniose. In the presence of glucose araCpBAD shows a very low basal expression level and therefore prevents GFP-expression.
  
 
===Functional Parameters===
 
===Functional Parameters===

Revision as of 05:49, 25 September 2015

araCpBad-GFP

Composite part consisting of an araC-regulated pBAD-promoter (BBa_K808000) cloned upstream of a ribosome binding site (BBa_B0034) and GFP (BBa_E0040). Because of the araC-regulated pBAD-promoter production of the GFP is induced by the addition of arabniose. In the presence of glucose araCpBAD shows a very low basal expression level and therefore prevents GFP-expression.

Functional Parameters

References

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1879
    Illegal SapI site found at 961