Difference between revisions of "Help:Plasmid backbones/Nomenclature"
| Line 42: | Line 42: | ||
===Version number=== | ===Version number=== | ||
(used to indicate miscellaneous interesting changes to backbone) | (used to indicate miscellaneous interesting changes to backbone) | ||
| − | *0 = | + | *0 = absent or incomplete BioBricks™ cloning site |
| − | *1 = complete | + | *1 = complete BioBricks™ cloning site (BCS) |
| − | *2 = 5’ terminator and | + | *2 = 5’ terminator and BCS |
| − | *3 = 5’ terminator and | + | *3 = 5’ terminator and BCS and 3’ terminator |
| − | *4 = | + | *4 = pSB2K3-derived plasmid free of many restriction site (designed for T7.1 project) |
*5 = Second generation of cloning plasmid. See [http://openwetware.org/wiki/Synthetic_Biology:Vectors/pSB%2A%2A5_design] for details. | *5 = Second generation of cloning plasmid. See [http://openwetware.org/wiki/Synthetic_Biology:Vectors/pSB%2A%2A5_design] for details. | ||
*6 = Held for some vector versions that the Berkeley iGEM team has made. (Chris Anderson). | *6 = Held for some vector versions that the Berkeley iGEM team has made. (Chris Anderson). | ||
*7 = Held for some vector versions that the Davidson iGEM team has made. | *7 = Held for some vector versions that the Davidson iGEM team has made. | ||
| − | *10 = Screening plasmid 1.0 by Jason Kelly. See [http://openwetware.org/wiki/Endy:Screening_plasmid] for details. | + | *10 = Screening plasmid 1.0 by Josh Michener and Jason Kelly. See [http://openwetware.org/wiki/Endy:Screening_plasmid] for details. |
*11 = Held for a version of Jason's measurement plasmid | *11 = Held for a version of Jason's measurement plasmid | ||
*12 = Held for a version of Jason's measurement plasmid | *12 = Held for a version of Jason's measurement plasmid | ||
Revision as of 00:15, 9 May 2007
Plasmids in the Registry of Standard Biological parts adhere to a particular naming convention. This page describes the details of this naming scheme.
If you are interested in designing and/or constructing a new BioBricks compatible plasmid and are submitting it to the Registry, please follow this naming convention. Questions or comments can be directed to Reshma Shetty.
Contents
pSBxyz-a
pSB = plasmid Synthetic Biology
x = origin of replication
y = selection marker
z = version number
-a = insert
Origin of replication
- 1= pUC19-derived pMB1 (in pSB series)
- 2 = pSCANS-derived
- 3 = p15A pMR101-derived
- 4 = rep101, repA pSC101-derived
- 5 = F plasmid
- 6 = pBR322
Selection marker
- X = none
- A = ampicillin
- C = chloramphenicol
- E = erythromycin
- G = gentamycin
- K = kanamycin
- N = neomycin
- Na = nalidixic acid
- R = rifampicin
- S = spectinomycin
- St = streptomycin
- T = tetracycline
- Tm = trimethoprim
- Z = zeocin
- AK = kanamycin and ampicillin
- KT = kanamycin and tetracycline
- AKT = ampicillin and kanamycin and tetracycline
- AC = ampicillin and chloramphenicol
… etc
Version number
(used to indicate miscellaneous interesting changes to backbone)
- 0 = absent or incomplete BioBricks™ cloning site
- 1 = complete BioBricks™ cloning site (BCS)
- 2 = 5’ terminator and BCS
- 3 = 5’ terminator and BCS and 3’ terminator
- 4 = pSB2K3-derived plasmid free of many restriction site (designed for T7.1 project)
- 5 = Second generation of cloning plasmid. See [http://openwetware.org/wiki/Synthetic_Biology:Vectors/pSB%2A%2A5_design] for details.
- 6 = Held for some vector versions that the Berkeley iGEM team has made. (Chris Anderson).
- 7 = Held for some vector versions that the Davidson iGEM team has made.
- 10 = Screening plasmid 1.0 by Josh Michener and Jason Kelly. See [http://openwetware.org/wiki/Endy:Screening_plasmid] for details.
- 11 = Held for a version of Jason's measurement plasmid
- 12 = Held for a version of Jason's measurement plasmid
- 20-29 = Held for Berkeley iGEM plasmids
Insert
- generally a BioBricks part number where multiple parts are separated by periods
- no dash = no insert
- -P1010 = ccd operon insert
- -1 = ccdB <-- this has now become legacy nomenclature.
Contact
Questions or comments can be directed to Reshma Shetty.