Difference between revisions of "Part:BBa K1602036"

Latest revision as of 02:45, 19 September 2015

Inducible generator of aes-SH3lig

This part is capable to generate the protein Acetyl Esterase (BBa_K1216002). To fuse the protein to an in vitro scaffold (BBa_K1602027) a linker-ligand sequence was added. This fusion could optimize the enzymatic degradation of Xylose by bringing the respective enzymes in spatial proximity. The ligand of this part binds to the SH3 domain of the scaffold.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 56
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 980

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-                 This part is capable to generate the protein Acetyl Esterase (<a href="https://parts.igem.org/Part:BBa_K1216002">BBa_K1216002</a>). To fuse the protein to an <i>in vitro</i> scaffold (<a href="https://parts.igem.org/Part:BBa_K1602027">BBa_K1602027</a>) a linker-ligand sequence was added. This fusion could optimize the enzymatic degradation of Xylose by bringing the respective enzymes in spatial proximity. The ligand of this part binds to the SH3 domain of the scaffold. The construct also contains a strong Ribosome Binding Site (<a href="https://parts.igem.org/Part:BBa_B0034">BBa_B0034</a>).
+                 This part is capable to generate the protein Acetyl Esterase (<a href="https://parts.igem.org/Part:BBa_K1216002">BBa_K1216002</a>). To fuse the protein to an <i>in vitro</i> scaffold (<a href="https://parts.igem.org/Part:BBa_K1602027">BBa_K1602027</a>) a linker-ligand sequence was added. This fusion could optimize the enzymatic degradation of Xylose by bringing the respective enzymes in spatial proximity. The ligand of this part binds to the SH3 domain of the scaffold.
                  </td>